用于检测抗肺炎球菌多糖抗原抗体的酶联免疫吸附测定：与放射免疫测定的比较。
Enzyme-linked immunosorbent assay for measurement of antibodies against pneumococcal polysaccharide antigens: comparison with radioimmunoassay.
作者信息
Callahan L T, Woodhour A F, Meeker J B, Hilleman M R
出版信息
J Clin Microbiol. 1979 Oct;10(4):459-63. doi: 10.1128/jcm.10.4.459-463.1979.
Abstract
An enzyme-linked immunosorbent assay (ELISA) for measuring antibodies against each of 14 polysaccharides in contemporary pneumococcal vaccine is described, and the findings of tests of paired sera from vaccinated human subjects are compared with those obtained by radioimmunoassay. The findings were in very poor agreement, and this appears to be due to the lesser ability of the ELISA procedure to measure antibody of low avidity. The ELISA procedure described here is not considered to be a satisfactory substitute for radioimmunoassay for measuring antibody responses to pneumococcal vaccine.
摘要
本文描述了一种用于检测当代肺炎球菌疫苗中14种多糖各自抗体的酶联免疫吸附测定（ELISA）方法，并将接种疫苗的人类受试者配对血清的检测结果与放射免疫测定法获得的结果进行了比较。结果显示两者一致性很差，这似乎是由于ELISA方法检测低亲和力抗体的能力较弱。本文所述的ELISA方法不被认为是替代放射免疫测定法来检测肺炎球菌疫苗抗体反应的令人满意的方法。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A quantitative immunochemical measure of the primary interaction between I BSA and antibody.一种对牛血清白蛋白（BSA）与抗体之间主要相互作用的定量免疫化学测定方法。
J Infect Dis. 1958 Nov-Dec;103(3):239-62. doi: 10.1093/infdis/103.3.239.
3
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
Immunochemistry. 1971 Sep;8(9):871-4. doi: 10.1016/0019-2791(71)90454-x.
4
The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity.酶联免疫吸附测定（ELISA）：一种抗体浓度或亲和力的测量方法。
Immunochemistry. 1978 Feb;15(2):131-6. doi: 10.1016/0161-5890(78)90053-6.
5
Serological diagnosis of pneumococcal disease with enzyme-linked immunosorbent assay (ELISA).采用酶联免疫吸附测定法（ELISA）对肺炎球菌疾病进行血清学诊断。
Scand J Infect Dis. 1978;10(3):177-81. doi: 10.3109/inf.1978.10.issue-3.04.
6
Studies in human subjects of polyvalent pneumococcal vaccines (39894).多价肺炎球菌疫苗的人体研究（39894）
Proc Soc Exp Biol Med. 1977 Oct;156(1):144-50. doi: 10.3181/00379727-156-39894.
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