Callahan L T, Woodhour A F, Meeker J B, Hilleman M R
J Clin Microbiol. 1979 Oct;10(4):459-63. doi: 10.1128/jcm.10.4.459-463.1979.
An enzyme-linked immunosorbent assay (ELISA) for measuring antibodies against each of 14 polysaccharides in contemporary pneumococcal vaccine is described, and the findings of tests of paired sera from vaccinated human subjects are compared with those obtained by radioimmunoassay. The findings were in very poor agreement, and this appears to be due to the lesser ability of the ELISA procedure to measure antibody of low avidity. The ELISA procedure described here is not considered to be a satisfactory substitute for radioimmunoassay for measuring antibody responses to pneumococcal vaccine.
本文描述了一种用于检测当代肺炎球菌疫苗中14种多糖各自抗体的酶联免疫吸附测定（ELISA）方法，并将接种疫苗的人类受试者配对血清的检测结果与放射免疫测定法获得的结果进行了比较。结果显示两者一致性很差，这似乎是由于ELISA方法检测低亲和力抗体的能力较弱。本文所述的ELISA方法不被认为是替代放射免疫测定法来检测肺炎球菌疫苗抗体反应的令人满意的方法。
