14价肺炎球菌荚膜多糖疫苗接种前后，酶联免疫吸附测定法（ELISA）和放射免疫测定法（RIA）检测肺炎球菌抗体的比较
Comparison of ELISA and RIA for measurement of pneumococcal antibodies before and after vaccination with 14-valent pneumococcal capsular polysaccharide vaccine.
作者信息
Koskela M, Leinonen M
出版信息
J Clin Pathol. 1981 Jan;34(1):93-8. doi: 10.1136/jcp.34.1.93.
Abstract
Antibody responses to the 14-valent pneumococcal capsular polysaccharide vaccine in children under school age were measured by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Specific IgG and IgM antibodies were usually detectable by ELISA in the prevaccination sera, and one or both of them increased as a response to the vaccination. Specific IGA antibodies were detected by ELISA in a part of the post-vaccination sera only. The frequency of the IgA responses increased with the age of the children. The correlation of the ELISA results with RIA was good (r from 0.652 to 0.812) except for type 6A (r = 0.471).
摘要
采用酶联免疫吸附测定（ELISA）和放射免疫测定（RIA）检测学龄期以下儿童对14价肺炎球菌荚膜多糖疫苗的抗体反应。接种疫苗前的血清中，通常可通过ELISA检测到特异性IgG和IgM抗体，接种疫苗后其中一种或两种抗体均会增加。仅在部分接种疫苗后的血清中通过ELISA检测到特异性IgA抗体。IgA反应的频率随儿童年龄增加而升高。除6A型（r = 0.471）外，ELISA结果与RIA的相关性良好（r为0.652至0.812）。
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