Fridlender B, Weissbach A
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3116-9. doi: 10.1073/pnas.68.12.3116.
The DNA polymerase enzymes from avian, murine, and feline RNA tumor viruses can be distinguished by their ability to read specific, synthetic primertemplates. The copying of templates containing adenylic and thymidylic acids by all these DNA polymerases is inhibited by ethidium bromide, though this compound affects the polymerases from mammalian tumor viruses much more than the enzyme from avian tumor viruses. Conversely, ethidium bromide stimulates the ability of the enzymes from avian tumor viruses to use primertemplates containing only guanylic and cytidylic acids, whereas the mammalian tumor virus enzymes are moderately inhibited.
禽、鼠和猫RNA肿瘤病毒的DNA聚合酶可通过其读取特定合成引物模板的能力来区分。所有这些DNA聚合酶对含有腺苷酸和胸苷酸的模板的复制都会受到溴化乙锭的抑制,不过该化合物对哺乳动物肿瘤病毒的聚合酶的影响比对禽肿瘤病毒的酶的影响要大得多。相反,溴化乙锭会刺激禽肿瘤病毒的酶使用仅含鸟苷酸和胞苷酸的引物模板的能力,而哺乳动物肿瘤病毒的酶则会受到适度抑制。