肿瘤病毒的DNA聚合酶：溴化乙锭对不同合成模板使用的特异性影响
DNA polymerases of tumor virus: specific effect of ethidium bromide on the use of different synthetic templates.
作者信息
Fridlender B, Weissbach A
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3116-9. doi: 10.1073/pnas.68.12.3116.
Abstract
The DNA polymerase enzymes from avian, murine, and feline RNA tumor viruses can be distinguished by their ability to read specific, synthetic primertemplates. The copying of templates containing adenylic and thymidylic acids by all these DNA polymerases is inhibited by ethidium bromide, though this compound affects the polymerases from mammalian tumor viruses much more than the enzyme from avian tumor viruses. Conversely, ethidium bromide stimulates the ability of the enzymes from avian tumor viruses to use primertemplates containing only guanylic and cytidylic acids, whereas the mammalian tumor virus enzymes are moderately inhibited.
摘要
禽、鼠和猫RNA肿瘤病毒的DNA聚合酶可通过其读取特定合成引物模板的能力来区分。所有这些DNA聚合酶对含有腺苷酸和胸苷酸的模板的复制都会受到溴化乙锭的抑制，不过该化合物对哺乳动物肿瘤病毒的聚合酶的影响比对禽肿瘤病毒的酶的影响要大得多。相反，溴化乙锭会刺激禽肿瘤病毒的酶使用仅含鸟苷酸和胞苷酸的引物模板的能力，而哺乳动物肿瘤病毒的酶则会受到适度抑制。
相似文献
1
DNA polymerases of tumor virus: specific effect of ethidium bromide on the use of different synthetic templates.肿瘤病毒的DNA聚合酶：溴化乙锭对不同合成模板使用的特异性影响
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3116-9. doi: 10.1073/pnas.68.12.3116.
2
Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses.致癌RNA病毒中DNA指导的DNA聚合酶产物的特性分析
Nature. 1970 Aug 8;227(5258):563-7. doi: 10.1038/227563a0.
3
DNA-directed DNA polymerase activity in oncogenic RNA viruses.致癌RNA病毒中的DNA指导的DNA聚合酶活性。
Nature. 1970 Sep 5;227(5262):1029-31. doi: 10.1038/2271029a0.
4
The inhibition of avian myeloblastosis virus deoxyribonucleic acid polymerase by synthetic polynucleotides.合成多核苷酸对禽成髓细胞瘤病毒脱氧核糖核酸聚合酶的抑制作用。
Biochemistry. 1974 Apr 23;13(9):1987-93. doi: 10.1021/bi00706a032.
5
RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.五种RNA病毒中的RNA依赖性DNA聚合酶活性：二价阳离子需求
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1789-96. doi: 10.1073/pnas.67.4.1789.
6
DNA polymerases from RNA tumor viruses and human cells: inhibition by polyuridylic acid.来自RNA肿瘤病毒和人类细胞的DNA聚合酶：多聚尿苷酸对其的抑制作用
Science. 1972 Sep 22;177(4054):1111-4. doi: 10.1126/science.177.4054.1111.
7
Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses.合成的DNA-RNA杂交体和RNA-RNA双链体作为致癌RNA病毒聚合酶的模板。
Nature. 1970 Oct 31;228(5270):430-2. doi: 10.1038/228430a0.
8
RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction.RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。II. 反应中RNA的直接导向作用。
J Virol. 1972 Jan;9(1):130-42. doi: 10.1128/JVI.9.1.130-142.1972.
9
10
Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin.细胞和病毒来源的核糖核酸酶H及DNA聚合酶对DNA-RNA杂交体的降解作用
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3360-4. doi: 10.1073/pnas.69.11.3360.
引用本文的文献
1
Differential sensitivity to ethidium bromide of replicative DNA synthesis and bleomycin-induced unscheduled DNA synthesis in permeable mouse sarcoma cells.通透的小鼠肉瘤细胞中复制性DNA合成和博来霉素诱导的非预定DNA合成对溴化乙锭的敏感性差异
Experientia. 1984 Aug 15;40(8):869-71. doi: 10.1007/BF01952001.
2
Characterization of RNase H activity associated with reverse transcriptase in simian foamy virus type 1.1型猿猴泡沫病毒中与逆转录酶相关的核糖核酸酶H活性的特性分析。
J Virol. 1983 Jul;47(1):249-52. doi: 10.1128/JVI.47.1.249-252.1983.
3
Studies on vaccinia virus-directed deoxyribonucleic acid polymerase.痘苗病毒导向的脱氧核糖核酸聚合酶的研究
J Virol. 1972 Oct;10(4):721-9. doi: 10.1128/JVI.10.4.721-729.1972.
4
A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes).一种来自人组织培养细胞的新型合成RNA依赖性DNA聚合酶（海拉细胞-成纤维细胞-合成寡核苷酸-模板-纯化酶）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):452-5. doi: 10.1073/pnas.69.2.452.
5
Influence of some antitumor antibiotics on viral neoplasia. The role of molecular structure in the inhibition of DNA polymerases from RNA tumor viruses, viral multiplication and tumor growth by some antitumor antibiotics.某些抗肿瘤抗生素对病毒性肿瘤形成的影响。分子结构在某些抗肿瘤抗生素抑制RNA肿瘤病毒的DNA聚合酶、病毒增殖及肿瘤生长中的作用。
Naturwissenschaften. 1972 Oct;59(10):448-55. doi: 10.1007/BF00592879.
6
Effects of (-)-2'-deoxy-3'-thiacytidine (3TC) 5'-triphosphate on human immunodeficiency virus reverse transcriptase and mammalian DNA polymerases alpha, beta, and gamma.（-）-2'-脱氧-3'-硫代胞苷三磷酸（3TC）对人类免疫缺陷病毒逆转录酶以及哺乳动物DNA聚合酶α、β和γ的影响
Antimicrob Agents Chemother. 1992 Aug;36(8):1688-94. doi: 10.1128/AAC.36.8.1688.
7
Requirement for cellular protein synthesis in reversal of ethidium-bormide-induced inhibition of cell transformation by murine sarcoma virus.细胞蛋白质合成在逆转溴化乙锭诱导的小鼠肉瘤病毒对细胞转化的抑制中的作用
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4337-40. doi: 10.1073/pnas.72.11.4337.
本文引用的文献
1
Inhibitors acting on nucleic acid synthesis in an oncogenic RNA virus.作用于致癌RNA病毒核酸合成的抑制剂。
Nat New Biol. 1971 Aug 4;232(31):143-5. doi: 10.1038/newbio232143a0.
2
Resonance energy transfer between ethidium bromide molecules bound to nucleic acids. Does intercalation wind or unwind the DNA helix?与核酸结合的溴化乙锭分子间的共振能量转移。嵌入作用会使DNA螺旋缠绕还是解旋？
J Mol Biol. 1971 Jul 14;59(1):43-62. doi: 10.1016/0022-2836(71)90412-8.
3
DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization.来自海拉细胞核的依赖DNA的DNA聚合酶。II. 模板和底物的利用
Biochem Biophys Res Commun. 1971 Aug 20;44(4):879-85. doi: 10.1016/0006-291x(71)90793-5.
4
DNA polymerase activities in varions of visna virus, a causative agent of a "slow" neurological disease.维斯纳病毒（一种“缓慢”神经疾病的病原体）病毒粒子中的DNA聚合酶活性。
Proc Natl Acad Sci U S A. 1971 Jan;68(1):182-6. doi: 10.1073/pnas.68.1.182.
5
Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases.区分RNA肿瘤病毒的逆转录酶与其他已知的DNA聚合酶。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2203-6. doi: 10.1073/pnas.68.9.2203.
6
Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses.RNA肿瘤病毒DNA聚合酶的引物需求和模板特异性
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507-11. doi: 10.1073/pnas.68.7.1507.
7
Inhibitors of the RNA and DNA dependent polymerase activities of RNA tumour viruses.RNA肿瘤病毒的RNA和DNA依赖性聚合酶活性抑制剂。
Nat New Biol. 1971 Jan 27;229(4):111-4. doi: 10.1038/newbio229111a0.
8
Inhibitors of DNA polymerases of murine leukemia viruses: activity of ethidium bromide.鼠白血病病毒DNA聚合酶抑制剂：溴化乙锭的活性
Science. 1971 Jul 30;173(3995):441-3. doi: 10.1126/science.173.3995.441.
9
RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.五种RNA病毒中的RNA依赖性DNA聚合酶活性：二价阳离子需求
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1789-96. doi: 10.1073/pnas.67.4.1789.
10
Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses.合成的DNA-RNA杂交体和RNA-RNA双链体作为致癌RNA病毒聚合酶的模板。
Nature. 1970 Oct 31;228(5270):430-2. doi: 10.1038/228430a0.
Zotero 插件