Redfield D C, Richman D D, Oxman M N, Kronenberg L H
Infect Immun. 1981 Jun;32(3):1216-26. doi: 10.1128/iai.32.3.1216-1226.1981.
Psoralen compounds covalently bind to nucleic acids when irradiated with long-wavelength ultraviolet light. This treatment can destroy the infectivity of deoxyribonucleic acid and ribonucleic acid viruses. Two psoralen compounds, 4'-hydroxymethyltrioxsalen and 4'-aminomethyltrioxsalen, were used with long-wavelength ultraviolet light to inactivate cell-free herpes simplex and influenza viruses and to render virus-infected cells noninfectious. This method of inactivation was compared with germicidal (short-wavelength) ultraviolet light irradiation. The antigenicity of the treated, virus-infected, antigen-bearing cells was examined by immunofluorescence and radioimmunoassay and by measuring the capacity of the herpes simplex virus-infected cells to stimulate virus-specific lymphocyte proliferation. The infectivity of the virus-infected cells could be totally eliminated without altering their viral antigenicity. The use of psoralen plus long-wavelength ultraviolet light is well suited to the preparation of noninfectious virus antigens and virus antigen-bearing cells for immunological assays.
补骨脂素化合物在长波紫外线照射下会与核酸共价结合。这种处理可破坏脱氧核糖核酸和核糖核酸病毒的感染性。两种补骨脂素化合物，即4'-羟甲基三甲沙林和4'-氨甲基三甲沙林，与长波紫外线一起用于使无细胞单纯疱疹病毒和流感病毒失活，并使病毒感染细胞失去感染性。将这种失活方法与杀菌（短波）紫外线照射进行了比较。通过免疫荧光和放射免疫测定法，以及通过测量单纯疱疹病毒感染细胞刺激病毒特异性淋巴细胞增殖的能力，来检测经处理的、病毒感染的、携带抗原的细胞的抗原性。病毒感染细胞的感染性可以被完全消除，而不会改变其病毒抗原性。补骨脂素加长效紫外线非常适合制备用于免疫测定的无感染性病毒抗原和携带病毒抗原的细胞。
