与纯化的猴病毒40病毒粒子相关的核酸内切酶活性。
Endonuclease activity associated with purified simian virus 40 virions.
作者信息
Kaplan J C, Wilbert S M, Black P H
出版信息
J Virol. 1972 May;9(5):800-3. doi: 10.1128/JVI.9.5.800-803.1972.
Abstract
Purified simian virus 40 has associated with it an endonuclease activity which converts form I (double-stranded, circular) simian virus 40 deoxyribonucleic acid to a nicked form that sediments as a homogeneous peak in alkaline sucrose gradients. The enzyme is dependent on magnesium ions for activity and is completely inhibited by ethylenediaminetetraacetic acid (0.02 m) or heat (80 C for 10 min). In tris(hydroxymethyl)aminomethane-hydrochloride buffer it exhibits optimal activity between pH 6.7 and 7.1 at 37 C. Gel electrophoretic analysis of purified, disrupted virus indicates the absence of detectable host cell protein contamination.
摘要
纯化的猴病毒40与一种核酸内切酶活性相关，该酶可将I型（双链、环状）猴病毒40脱氧核糖核酸转化为带切口的形式，这种形式在碱性蔗糖梯度中以单一峰的形式沉降。该酶的活性依赖于镁离子，并且完全被乙二胺四乙酸（0.02 m）或加热（80℃处理10分钟）所抑制。在三（羟甲基）氨基甲烷 - 盐酸盐缓冲液中，它在37℃、pH 6.7至7.1之间表现出最佳活性。对纯化的、裂解的病毒进行凝胶电泳分析表明，未检测到可检测到的宿主细胞蛋白污染。
相似文献
1
Endonuclease activity associated with purified simian virus 40 virions.与纯化的猴病毒40病毒粒子相关的核酸内切酶活性。
J Virol. 1972 May;9(5):800-3. doi: 10.1128/JVI.9.5.800-803.1972.
2
Characterization of an endonuclease associated with simian virus 40 virions.与猿猴病毒40病毒粒子相关的一种核酸内切酶的特性分析。
J Virol. 1972 Sep;10(3):410-6. doi: 10.1128/JVI.10.3.410-416.1972.
3
A study on the cleavage site specificity of the endonuclease associated with purified simian virus 40 particles.
FEBS Lett. 1973 Aug 15;34(2):193-7. doi: 10.1016/0014-5793(73)80791-4.
4
Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.由埃及嗜血杆菌的一种限制酶——核酸内切酶Z产生的φX174脱氧核糖核酸的特定片段。
J Virol. 1972 Jul;10(1):42-50. doi: 10.1128/JVI.10.1.42-50.1972.
5
Simian virus 40 deoxyribonucleic acid synthesis: analysis by gel electrophoresis.猴病毒40脱氧核糖核酸合成：通过凝胶电泳进行分析
J Virol. 1972 Oct;10(4):599-604. doi: 10.1128/JVI.10.4.599-604.1972.
6
A convenient micro method for the quantitation of closed circular deoxyribonucleic acid.一种用于定量闭环脱氧核糖核酸的便捷微量方法。
Biochemistry. 1974 May 21;13(11):2410-3. doi: 10.1021/bi00708a027.
7
Deoxyribonucleic acid replication in simian virus 40-infected cells. II. Detection and characterization of simian virus 40 pseudovirions.猿猴病毒40感染细胞中的脱氧核糖核酸复制。II. 猿猴病毒40假病毒颗粒的检测与特性分析。
J Virol. 1970 Apr;5(4):451-7. doi: 10.1128/JVI.5.4.451-457.1970.
8
Endonuclease activity associated with purified PM2 bacteriophages.与纯化的PM2噬菌体相关的核酸内切酶活性。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4965-9. doi: 10.1073/pnas.71.12.4965.
9
Intracellular forms of adenovirus deoxyribonucleic acid. I. Evidence for a deoxyribonucleic acid-protein complex in baby hamster kidney cells infected with adenovirus type 12.腺病毒脱氧核糖核酸的细胞内形式。I. 感染12型腺病毒的幼仓鼠肾细胞中存在脱氧核糖核酸-蛋白质复合物的证据。
J Virol. 1972 Feb;9(2):297-308. doi: 10.1128/JVI.9.2.297-308.1972.
10
Structural polypeptides of simian virus 40.猴病毒40的结构多肽
J Virol. 1971 May;7(5):635-41. doi: 10.1128/JVI.7.5.635-641.1971.
引用本文的文献
1
Interactions of Polyoma and Mouse DNAs III. Mechanism of Polyoma Pseudovirion Formation.多瘤病毒与小鼠DNA的相互作用III. 多瘤病毒假病毒体形成机制
J Virol. 1975 May;15(5):1158-67. doi: 10.1128/JVI.15.5.1158-1167.1975.
2
Fibroma virus-induced acid deoxyribonuclease in rabbit kidney cells.
Arch Gesamte Virusforsch. 1974;44(1):14-22. doi: 10.1007/BF01242176.
3
Endonuclease activity associated with purified PM2 bacteriophages.与纯化的PM2噬菌体相关的核酸内切酶活性。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4965-9. doi: 10.1073/pnas.71.12.4965.
4
Characterization of an endonuclease associated with simian virus 40 virions.与猿猴病毒40病毒粒子相关的一种核酸内切酶的特性分析。
J Virol. 1972 Sep;10(3):410-6. doi: 10.1128/JVI.10.3.410-416.1972.
5
Early events in cell-animal virus interactions.细胞与动物病毒相互作用的早期事件。
Bacteriol Rev. 1973 Jun;37(2):103-35. doi: 10.1128/br.37.2.103-135.1973.
6
Canine systemic lupus erythematosus. Transmission of serologic abnormalities by cell-free filtrates.犬全身性红斑狼疮。无细胞滤液介导的血清学异常传递。
J Clin Invest. 1973 Aug;52(8):1893-907. doi: 10.1172/JCI107373.
7
Ribonuclease activities associated with purified foot and mouth disease virus.
Arch Virol. 1978;57(2):153-9. doi: 10.1007/BF01315676.
8
Endonucleases and simian virus 40 virions: origin of a virion-associated endonuclease.核酸内切酶与猿猴病毒40病毒粒子：一种病毒粒子相关核酸内切酶的起源
J Virol. 1976 Feb;17(2):352-62. doi: 10.1128/JVI.17.2.352-362.1976.
9
Origin of the polyoma virus-associated endonuclease.多瘤病毒相关核酸内切酶的起源。
J Virol. 1975 Jan;17(1):127-31. doi: 10.1128/JVI.17.1.127-131.1976.
本文引用的文献
1
THE NUCLEIC ACID OF SIMIAN VIRUS 40.猴病毒40的核酸
Virology. 1964 Nov;24:388-92. doi: 10.1016/0042-6822(64)90176-x.
2
THE PURIFICATION OF SIMIAN VIRUS 40.猿猴病毒40的纯化
Virology. 1964 Nov;24:381-7. doi: 10.1016/0042-6822(64)90175-8.
3
Evidence for a new endonuclease synthesized by lambda bacteriophage.λ噬菌体合成新的核酸内切酶的证据。
J Virol. 1968 Oct;2(10):1096-101. doi: 10.1128/JVI.2.10.1096-1101.1968.
4
Adenovirus endonuclease: association with the penton of adenovirus type 2.腺病毒核酸内切酶：与2型腺病毒五邻体的关联。
J Mol Biol. 1971 Aug 28;60(1):45-64. doi: 10.1016/0022-2836(71)90446-3.
5
Two deoxyribonuclease activities within purified vaccinia virus.纯化牛痘病毒中的两种脱氧核糖核酸酶活性。
Proc Natl Acad Sci U S A. 1969 Jul;63(3):820-7. doi: 10.1073/pnas.63.3.820.
6
Analysis of simian virus 40-induced transformation of hamster kidney tissue in vitro. 8. Induction of infectious simian virus 40 from virogenie transformed hamster cells by amino acid deprivation or cycloheximide treatment.体外分析猿猴病毒40诱导的仓鼠肾组织转化。8. 通过氨基酸剥夺或环己酰亚胺处理从病毒基因转化的仓鼠细胞中诱导出传染性猿猴病毒40 。
J Virol. 1972 Mar;9(3):448-53. doi: 10.1128/JVI.9.3.448-453.1972.
7
[Endonucleasic activity of purified preparations of Polyoma virus].
C R Acad Hebd Seances Acad Sci D. 1971 Dec 20;273(25):2650-3.
8
Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of primary infected Chinese hamster cells.猴病毒40脱氧核糖核酸整合到初次感染的中国仓鼠细胞的脱氧核糖核酸中。
J Virol. 1971 Nov;8(5):708-15. doi: 10.1128/JVI.8.5.708-715.1971.
9
Deoxyribonucleic acid polymerase(s) of Rous sarcoma virus: effects of virion-associated endonuclease on the enzymatic product.劳氏肉瘤病毒的脱氧核糖核酸聚合酶：病毒体相关核酸内切酶对酶促产物的影响。
J Virol. 1971 Jul;8(1):17-27. doi: 10.1128/JVI.8.1.17-27.1971.
10
Structural polypeptides of simian virus 40.猴病毒40的结构多肽
J Virol. 1971 May;7(5):635-41. doi: 10.1128/JVI.7.5.635-641.1971.
Zotero 插件