Gregory J F, Kirk J R
Am J Clin Nutr. 1979 Apr;32(4):879-83. doi: 10.1093/ajcn/32.4.879.
A high performance liquid chromatographic method is presented for the determination of the vitamin B6 metabolite, 4-pyridoxic acid, in urine. Urine samples are treated with trichloroacetic acid to precipitate protein. An aliquot of the supernatant is chromatographed using 0.033 M phosphate buffer containing 5% (v/v) methanol (pH 2.2), a fluorometric detector and a commercial reverse phase octadecylsilica column. The high precision of the method and the absence of interfering compounds have been demonstrated. This method provides a rapid, sensitive, and quantitative technique for the measurement of urinary 4-pyridoxic acid for use in metabolic and nutritional studies.
本文介绍了一种用高效液相色谱法测定尿液中维生素B6代谢物4-吡哆酸的方法。尿液样本用三氯乙酸处理以沉淀蛋白质。取上清液的一份等分试样，使用含有5%（v/v）甲醇（pH 2.2）的0.033 M磷酸盐缓冲液、荧光检测器和市售反相十八烷基硅胶柱进行色谱分析。该方法已证明具有高精度且不存在干扰化合物。该方法为代谢和营养研究中测定尿中4-吡哆酸提供了一种快速、灵敏且定量的技术。
