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从劳斯肉瘤病毒转化的哺乳动物细胞中拯救劳斯肉瘤病毒。

Rescue of rous sarcoma virus from rous sarcoma virus-transformed mammalian cells.

作者信息

Coffin J M

出版信息

J Virol. 1972 Jul;10(1):153-6. doi: 10.1128/JVI.10.1.153-156.1972.

Abstract

Rat cells transformed by the B77 strain of avian sarcoma virus produce no virus-like particles, yet B77 virus was rescued from these cells by Sendai virus-mediated fusion with chicken cells. This virus rescue was not affected by treatment of the chicken cells with agents that rendered the cells incapable of dividing, although such treatment greatly reduced the ability of the chicken cells to plate as infectious centers after infection with B77 virus. Fusion of R(B77) cells with chicken erythrocytes also led to virus rescue, although with less efficiency than fusion with chicken fibroblasts. Therefore, virus rescue was probably due to a factor or factors contributed by chicken cells which aid in virus production.

摘要

由禽肉瘤病毒B77株转化的大鼠细胞不产生病毒样颗粒,但通过仙台病毒介导与鸡细胞融合,从这些细胞中拯救出了B77病毒。这种病毒拯救不受用使细胞无法分裂的试剂处理鸡细胞的影响,尽管这种处理大大降低了鸡细胞在感染B77病毒后作为感染中心形成集落的能力。R(B77)细胞与鸡红细胞融合也导致了病毒拯救,尽管效率低于与鸡成纤维细胞融合。因此,病毒拯救可能是由于鸡细胞贡献的一种或多种因子有助于病毒产生。

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