Steimer K S, Boettiger D
J Virol. 1977 Jul;23(1):133-41. doi: 10.1128/JVI.23.1.133-141.1977.
Polyethylene glycol (PEG) is effective as a fusing agent for the rescue of virus from Rous sarcoma virus-transformed mammalian cells. The procedure of PEG-mediated rescue of virus from virogenic cell lines is described, and the technique is compared with that of Sendai virus-mediated rescue. Virus may be rescued quantitatively from virogenic cell lines by plating mitomycin C-killed transformed mammalian cells with chicken embryo cells, treating the monolayers with 50% PEG and overlaying the monolayers with focus agar. The number of foci that appeared reflected the number of heterokaryons in the fusion mixtures that released infectious virus. PEG gave reproducible results in virus rescue experiments with an efficiency equal to the best Sendai virus preparations. In addition to the description of the technique for PEG-mediated virus rescue from virogenic cell lines, a method for virus rescue from nonvirogenic lines is presented. Preinfection of the chicken embryo cells with helper avian leukosis virus (Rous-associated virus) prior to fusion with mammalian cells transformed by defective viruses complements the virus defect. We examined four nonvirogenic cell lines, and all released infectious virus in the complementation rescue assay.
聚乙二醇（PEG）作为一种融合剂，在从劳斯肉瘤病毒转化的哺乳动物细胞中拯救病毒方面是有效的。本文描述了通过PEG介导从产病毒细胞系中拯救病毒的方法，并将该技术与仙台病毒介导的拯救技术进行了比较。通过将丝裂霉素C处理过的转化哺乳动物细胞与鸡胚细胞共同培养，用50% PEG处理单层细胞，并在单层细胞上覆盖焦点琼脂，可从产病毒细胞系中定量拯救病毒。出现的病灶数量反映了释放感染性病毒的融合混合物中异核体的数量。在病毒拯救实验中，PEG产生了可重复的结果，其效率与最佳的仙台病毒制剂相当。除了描述通过PEG介导从产病毒细胞系中拯救病毒的技术外，还介绍了一种从非产病毒细胞系中拯救病毒的方法。在用缺陷病毒转化的哺乳动物细胞融合之前，先用辅助禽白血病病毒（劳斯相关病毒）感染鸡胚细胞，可弥补病毒缺陷。我们检测了四个非产病毒细胞系，在互补拯救试验中，所有细胞系都释放了感染性病毒。
