前列腺素E1和F2对离体兔耳灌注血管的作用。

The actions of prostaglandins E 1 and F 2 on the on the perfused vessels of the isolated rabbit ear.

al-Tai S A, Graham J D

Br J Pharmacol. 1972 Apr;44(4):699-710. doi: 10.1111/j.1476-5381.1972.tb07308.x.

Abstract

In the isolated rabbit ear vascular bed, perfused with Krebs solution, prostaglandins E(1) and F(2alpha) produce dose-dependent, phentolamine-sensitive constrictions.2. These are absent if the animal is pre-treated with reserpine or if the ear is denervated in advance.3. If noradrenaline or vasopressin is added to the Krebs solution, vascular resistance is high and PGE(1) and PGF(2alpha) produce vasodilatation which is unaffected by hyoscine or propranolol.4. Perfusion with theophylline, with added ATP, ADP or 3'5'-AMP, or pre-treatment of the animal with stilboestrol antagonizes the dilator response to PGE(1) in the presence of noradrenaline, which may be reversed. Most of the responses to PGF(2alpha) are reversed. These treatments elevate the level of 3'5'-AMP in tissues.5. It is postulated that prostaglandins exert a regulatory action on 3'5'-AMP levels through inhibition of adenyl cyclase and/or phosphodiesterase and that the resulting rising or falling level of 3'5'-AMP determines the nature of the response by the smooth muscle to the released noradrenaline.

摘要

在灌注有 Krebs 溶液的离体兔耳血管床中，前列腺素 E(1) 和 F(2α) 产生剂量依赖性、酚妥拉明敏感的收缩作用。
如果动物预先用利血平处理或耳朵预先去神经支配，则不存在这些收缩作用。
如果将去甲肾上腺素或血管加压素添加到 Krebs 溶液中，血管阻力会升高，PGE(1) 和 PGF(2α) 会产生血管舒张作用，而这种作用不受东莨菪碱或普萘洛尔的影响。
用茶碱灌注，并添加 ATP、ADP 或 3',5'-AMP，或者用己烯雌酚对动物进行预处理，会拮抗在去甲肾上腺素存在下对 PGE(1) 的舒张反应，这种反应可能会被逆转。对 PGF(2α) 的大多数反应都会被逆转。这些处理会提高组织中 3',5'-AMP 的水平。
据推测，前列腺素通过抑制腺苷酸环化酶和/或磷酸二酯酶对 3',5'-AMP 水平发挥调节作用，并且由此导致的 3',5'-AMP 水平的升高或降低决定了平滑肌对释放的去甲肾上腺素的反应性质。