硝酸纤维素膜上猿猴病毒40 DNA-RNA杂交体的丢失；对病毒-宿主DNA相互作用研究的启示。
Loss of simian virus 40 DNA-RNA hybrids from nitrocellulose membranes; implications for the study of virus--host DNA interactions.
作者信息
Haas M, Vogt M, Dulbecco R
出版信息
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2160-4. doi: 10.1073/pnas.69.8.2160.
Abstract
Complete hybrids of simian virus 40 (SV40)DNA and its complementary RNA (cRNA) are not retained on nitrocellulose membranes. At saturating cRNA concentrations, retention of the hybrids indicates incomplete homology between DNA and RNA, probably due to incorporation of host DNA in the viral DNA; this effect is most pronounced when DNA is produced in cells infected at high multiplicity. Hybrids between DNA of Chinese hamster cells transformed by SV40 and cRNA are retained if the DNA fragments are long, but they are lost if the DNA is sheared to less than the length of an SV40 DNA molecule. Hence, in cells examined with about six SV40 genomes per cell, each genome is individually integrated. The results may explain previous discrepancies in the estimation of the number of viral genomes in transformed cells.
摘要
猿猴病毒40（SV40）DNA与其互补RNA（cRNA）的完全杂交体不会保留在硝酸纤维素膜上。在cRNA浓度饱和时，杂交体的保留表明DNA和RNA之间存在不完全同源性，这可能是由于宿主DNA掺入了病毒DNA中；当在高感染复数下感染的细胞中产生DNA时，这种效应最为明显。如果DNA片段较长，SV40转化的中国仓鼠细胞的DNA与cRNA之间的杂交体可以保留，但如果DNA被剪切成小于一个SV40 DNA分子的长度，则会丢失。因此，在用每个细胞约六个SV40基因组进行检测的细胞中，每个基因组都是单独整合的。这些结果可能解释了先前在估计转化细胞中病毒基因组数量时出现的差异。
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