使用与重氮苄氧基甲基纸共价连接的DNA对特定标记RNA进行定量分析。
Quantitative analysis of specific labelled RNA'S using DNA covalently linked to diazobenzyloxymethyl-paper.
作者信息
Stark G R, Williams J G
出版信息
Nucleic Acids Res. 1979 Jan;6(1):195-203. doi: 10.1093/nar/6.1.195.
Abstract
Substantial amounts of DNA (at least 25 microgram per cm2) can be stably bound to diazobenzyloxymethyl (DBM)-paper. Complementary RNA will hybridize to the DNA paper almost completely in 24 hours. Using several different conditions of hybridization and washing, the background of RNA bound non-specifically is very low (between 0.01 and 0.02%) and the efficiency of hybridization is very high (75 to 50% of complementary RNA is bound and retained through the washing procedure). Because the DNA is bound to the paper convalently, it is retained through all the washing and elution steps, and the DNA papers can be re-used many times.
摘要
大量的DNA（每平方厘米至少25微克）能够稳定地结合到重氮苄氧基甲基（DBM）纸上。互补RNA在24小时内几乎能与DNA纸完全杂交。使用几种不同的杂交和洗涤条件，非特异性结合的RNA背景非常低（在0.01%至0.02%之间），杂交效率非常高（75%至50%的互补RNA在洗涤过程中被结合并保留）。由于DNA与纸共价结合，它在所有洗涤和洗脱步骤中都能保留下来，并且DNA纸可以多次重复使用。
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本文引用的文献
1
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J Mol Biol. 1965 Jul;12(3):829-42. doi: 10.1016/s0022-2836(65)80331-x.
2
Transcription of ribonucleic acid in isolated mouse myeloma nuclei.分离的小鼠骨髓瘤细胞核中核糖核酸的转录
Biochemistry. 1973 Aug 28;12(18):3440-6. doi: 10.1021/bi00742a013.
3
Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells.猴病毒40的转录。I. 猴病毒40 DNA链的分离以及分离出的链与从裂解感染和转化细胞中提取的RNA的杂交。
J Mol Biol. 1972 Sep 14;70(1):57-71. doi: 10.1016/0022-2836(72)90163-5.
4
Loss of simian virus 40 DNA-RNA hybrids from nitrocellulose membranes; implications for the study of virus--host DNA interactions.硝酸纤维素膜上猿猴病毒40 DNA-RNA杂交体的丢失；对病毒-宿主DNA相互作用研究的启示。
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2160-4. doi: 10.1073/pnas.69.8.2160.
5
Selective extraction of polyoma DNA from infected mouse cell cultures.从受感染的小鼠细胞培养物中选择性提取多瘤病毒DNA。
J Mol Biol. 1967 Jun 14;26(2):365-9. doi: 10.1016/0022-2836(67)90307-5.
6
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