Lazdunski C, Shapiro B M
J Bacteriol. 1972 Aug;111(2):495-8. doi: 10.1128/jb.111.2.495-498.1972.
Mutants of Escherichia coli which have a defect in their permeability barrier were selected. The technique used was to employ a strain of E. coli having a deletion in the gene for lactose permease and to select for mutants which can grow on lactose at 40 C. Twenty such mutants were isolated and six of these were found to be more sensitive to actinomycin D, sodium deoxycholate, and sodium dodecyl sulfate than was the parental strain. They were also more sensitive to the antibiotics vancomycin and bacitracin, which inhibit peptidoglycan biosynthesis. These mutants were no more sensitive to several different colicins or phages than was the wild-type strain. One of the mutants selected by this technique has an abnormal morphology when grown on certain carbon sources in minimal medium, and this mutant is more extensively studied in the accompanying paper.
筛选出了在其通透性屏障方面存在缺陷的大肠杆菌突变体。所采用的技术是使用一株在乳糖通透酶基因上有缺失的大肠杆菌菌株，并筛选出能够在40℃下利用乳糖生长的突变体。分离出了20个这样的突变体，其中6个被发现比亲本菌株对放线菌素D、脱氧胆酸钠和十二烷基硫酸钠更敏感。它们对抑制肽聚糖生物合成的抗生素万古霉素和杆菌肽也更敏感。这些突变体对几种不同的大肠杆菌素或噬菌体的敏感性并不比野生型菌株更高。通过这种技术筛选出的一个突变体在基本培养基中的某些碳源上生长时具有异常形态，并且在随附的论文中对该突变体进行了更深入的研究。
