猴病毒40在有效感染和转化细胞中的转录。
Simian virus 40 transcription in productively infected and transformed cells.
作者信息
Weinberg R A, Ben-Ishai Z, Newbold J E
出版信息
J Virol. 1974 Jun;13(6):1263-73. doi: 10.1128/JVI.13.6.1263-1273.1974.
Abstract
Several independent cell lines transformed by simian virus 40 carry a species of viral RNA of 900,000 to 1,000,000 daltons. A viral RNA species of similar size is found early in the lytic cycle. Late in the viral lytic cycle, two prominent viral RNA species of about 600,000 and 900,000 daltons are seen. The larger late species shares nucleotide sequences with, and is less stable than, the smaller. These RNA species are located in the cytoplasm of the infected cell. The regions of the viral genome coding for these RNA species are mapped by hybridization of lytic RNA species to fragments of the genome produced by cleavage with Haemophilus aegyptius endonuclease.
摘要
几种由猿猴病毒40转化的独立细胞系带有一种分子量为900,000至1,000,000道尔顿的病毒RNA。在裂解周期早期发现了一种大小相似的病毒RNA。在病毒裂解周期后期，可见两种突出的病毒RNA，分子量约为600,000和900,000道尔顿。较大的后期RNA物种与较小的RNA物种共享核苷酸序列，且稳定性较差。这些RNA物种位于受感染细胞的细胞质中。通过将裂解RNA物种与埃及嗜血杆菌内切核酸酶切割产生的基因组片段进行杂交，绘制出编码这些RNA物种的病毒基因组区域。
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