Warnaar S O, De Mol A W
J Virol. 1973 Jul;12(1):124-9. doi: 10.1128/JVI.12.1.124-129.1973.
Two discrete simian virus 40 (SV40) RNA species sedimenting at 19 and 16S, respectively, that are present in infected BS-C-1 cells were characterized with respect to the base composition and the ribonuclease T1 fingerprints. The base composition of the 19S SV40 RNA was found to be cytidylic acid (C), 23.0; adenylic acid (A), 28.3; guanylic acid (G), 23.9; and uridylic acid (U), 24.8; that of the 16S SV40 RNA was C, 19.3; A, 34.0; G, 22.0; and U, 24.7 mol%. Analysis of the ribonuclease T1 fingerprints indicated a difference in the base sequence of the 19 and 16S SV40 RNA. The presence of long sequences of adenylic acid residues (poly A) in these viral RNAs was confirmed.
在感染的BS - C - 1细胞中存在的两种分别沉降系数为19S和16S的离散猴病毒40(SV40)RNA种类,就碱基组成和核糖核酸酶T1指纹图谱进行了表征。发现19S SV40 RNA的碱基组成为:胞苷酸(C),23.0;腺苷酸(A),28.3;鸟苷酸(G),23.9;尿苷酸(U),24.8;16S SV40 RNA的碱基组成为:C,19.3;A,34.0;G,22.0;U,24.7摩尔%。核糖核酸酶T1指纹图谱分析表明19S和16S SV40 RNA的碱基序列存在差异。证实了这些病毒RNA中存在长序列的腺苷酸残基(多聚A)。