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来自感染的BS-C-1细胞的两种猿猴病毒40特异性RNA分子的特性分析。

Characterization of two simian virus 40-specific RNA molecules from infected BS-C-1 cells.

作者信息

Warnaar S O, De Mol A W

出版信息

J Virol. 1973 Jul;12(1):124-9. doi: 10.1128/JVI.12.1.124-129.1973.

Abstract

Two discrete simian virus 40 (SV40) RNA species sedimenting at 19 and 16S, respectively, that are present in infected BS-C-1 cells were characterized with respect to the base composition and the ribonuclease T1 fingerprints. The base composition of the 19S SV40 RNA was found to be cytidylic acid (C), 23.0; adenylic acid (A), 28.3; guanylic acid (G), 23.9; and uridylic acid (U), 24.8; that of the 16S SV40 RNA was C, 19.3; A, 34.0; G, 22.0; and U, 24.7 mol%. Analysis of the ribonuclease T1 fingerprints indicated a difference in the base sequence of the 19 and 16S SV40 RNA. The presence of long sequences of adenylic acid residues (poly A) in these viral RNAs was confirmed.

摘要

在感染的BS - C - 1细胞中存在的两种分别沉降系数为19S和16S的离散猴病毒40(SV40)RNA种类,就碱基组成和核糖核酸酶T1指纹图谱进行了表征。发现19S SV40 RNA的碱基组成为:胞苷酸(C),23.0;腺苷酸(A),28.3;鸟苷酸(G),23.9;尿苷酸(U),24.8;16S SV40 RNA的碱基组成为:C,19.3;A,34.0;G,22.0;U,24.7摩尔%。核糖核酸酶T1指纹图谱分析表明19S和16S SV40 RNA的碱基序列存在差异。证实了这些病毒RNA中存在长序列的腺苷酸残基(多聚A)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ad/355238/71149119b7c5/jvirol00259-0135-a.jpg

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