球形红假单胞菌和D型着色菌提取物中的粪卟啉原酶活性。
Coproporphyrinogenase activities in extracts of Rhodopseudomonas spheroides and Chromatium strain D.
作者信息
Tait G H
出版信息
Biochem J. 1972 Aug;128(5):1159-69. doi: 10.1042/bj1281159.
Abstract
- The anaerobic coproporphyrinogenase activity in an extract of Rhodopseudomonas spheroides is inhibited by 1,10-phenanthroline, alphaalpha'-bipyridyl, flavins, 2,4-dinitrophenol and 1,4-naphthaquinone. These compounds have no effect on the aerobic coproporphyrinogenase activity. 2. On removal of small-molecular-weight material from a crude extract, the anaerobic system becomes very unstable; it can be stabilized by adding succinate. Now nicotinamide nucleotides, in addition to Mg(2+), ATP and methionine, are required for protoporphyrin to be formed. 3. A mechanism for the anaerobic reaction is proposed, based on the cofactor requirements and the effect of inhibitors. 4. The enzyme responsible for aerobic activity has been partially purified and some of its properties are reported. 5. A crude extract of Chromatium strain D also exhibits coproporphyrinogenase activity under anaerobic conditions in the presence of S-adenosylmethionine or ATP plus methionine. The requirement for other cofactors is variable.
摘要
- 球形红假单胞菌提取物中的厌氧粪卟啉原酶活性受到1,10 - 菲咯啉、α,α'-联吡啶、黄素、2,4 - 二硝基苯酚和1,4 - 萘醌的抑制。这些化合物对需氧粪卟啉原酶活性没有影响。2. 从粗提取物中去除小分子物质后，厌氧系统变得非常不稳定；加入琥珀酸盐可使其稳定。现在，除了Mg(2+)、ATP和蛋氨酸外，原卟啉的形成还需要烟酰胺核苷酸。3. 根据辅因子需求和抑制剂的作用，提出了厌氧反应的机制。4. 负责需氧活性的酶已部分纯化，并报道了其一些性质。5. 在厌氧条件下，当存在S - 腺苷甲硫氨酸或ATP加蛋氨酸时，嗜色菌D菌株的粗提取物也表现出粪卟啉原酶活性。对其他辅因子的需求是可变的。
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