MacLeod R A, Thurman P, Rogers H J
J Bacteriol. 1973 Jan;113(1):329-40. doi: 10.1128/jb.113.1.329-340.1973.
Sodium ion was shown to stimulate strongly the transport of l-glutamic acid into cells of Bacillus licheniformis 6346 His(-). Lithium ion had a slight capacity to replace Na(+) in this capacity, but K(+) was without effect. Three of five amino acids tested. l-glutamic acid, l-aspartic acid, and l-alanine, were concentrated against a gradient in the cells. Intracellular pools of these amino acids were extractable with 5% trichloroacetic acid. Pools of l-histidine and l-lysine could not be detected. No evidence of active transport of lysine into cells could be detected, and histidine was taken up in the absence of chloramphenicol but not in its presence. The uptake of glutamic acid by membrane vesicle preparations was strongly stimulated by reduced nicotinamide adenine dinucleotide (NADH) and to a lesser extent by succinate. The presence of phenazine methosulfate increased uptake in the presence of succinate. Either l- or d-lactate and adenosine triphosphate were without effect. None of these compounds stimulated the uptake of glutamic acid by mesosomes, although some mesosome preparations contained separable membrane which was very active. NADH strongly stimulated the uptake of aspartic acid and alanine by membrane vesicles but had only a slight effect on the uptake of histidine and lysine. No evidence of active transport of any of the amino acids into mesosomes could be detected either in the presence or absence of NADH. NADH stimulation of the uptake of glutamic acid by membrane vesicles was destroyed by exposure to light of 360 nm; this inactivation was reversible by vitamin K(2(5)) or K(2(10)). Sodium ion stimulated transport of glutamic acid by membrane vesicles.
已表明钠离子能强烈刺激L-谷氨酸转运到地衣芽孢杆菌6346 His(-)的细胞中。锂离子在该功能上有轻微的能力替代Na(+)，但钾离子没有作用。所测试的五种氨基酸中的三种，L-谷氨酸、L-天冬氨酸和L-丙氨酸，在细胞中逆浓度梯度被浓缩。这些氨基酸的细胞内池可用5%三氯乙酸提取。未检测到L-组氨酸和L-赖氨酸的池。未检测到赖氨酸主动转运到细胞中的证据，并且在没有氯霉素时组氨酸被摄取，但在有氯霉素时则不被摄取。膜囊泡制剂对谷氨酸的摄取受到还原型烟酰胺腺嘌呤二核苷酸（NADH）的强烈刺激，琥珀酸的刺激作用较小。在有琥珀酸存在时，吩嗪硫酸甲酯的存在增加了摄取。L-或D-乳酸和三磷酸腺苷均无作用。这些化合物均未刺激中间体对谷氨酸的摄取，尽管一些中间体制剂含有非常活跃的可分离膜。NADH强烈刺激膜囊泡对天冬氨酸和丙氨酸的摄取，但对组氨酸和赖氨酸的摄取仅有轻微影响。在有或没有NADH的情况下，均未检测到任何氨基酸主动转运到中间体中的证据。膜囊泡对谷氨酸摄取的NADH刺激作用在暴露于360nm光时被破坏；这种失活可被维生素K(2(5))或K(2(10))逆转。钠离子刺激膜囊泡对谷氨酸的转运。
