大根鬼伞中诱导子实体形成物质的纯化与鉴定
Purification and identification of the fruiting-inducing substances in Coprinus macrorhizus.
作者信息
Uno I, Ishikawa T
出版信息
J Bacteriol. 1973 Mar;113(3):1240-8. doi: 10.1128/jb.113.3.1240-1248.1973.
Abstract
Substances which are effective in inducing fruiting bodies in monokaryotic mycelia of the fis(+) strain of Coprinus macrorhizus were purified and characterized. The active components of fruiting-inducing substances were identified as adenosine-3'-monophosphate, adenosine 3',5'-cyclic monophosphate (cyclic AMP), and a protein which is bound with the cyclic AMP. Cyclic AMP was synthesized from adenine within mycelia of the mutant strains which form monokaryotic fruiting bodies without the addition of fruiting-inducing substances, but not in those of the strains which do not form monokaryotic fruiting bodies. The proteins which bind with cyclic AMP were detected in crude extracts of mycelia of those strains which form monokaryotic fruiting bodies and of the dikaryon, but not in those of the strains which do not form monokaryotic fruiting bodies.
摘要
对在大根鬼伞fis(+)菌株单核菌丝体中诱导子实体形成有效的物质进行了纯化和特性鉴定。诱导子实体形成物质的活性成分被鉴定为3'-单磷酸腺苷、3',5'-环磷酸腺苷（环磷腺苷）以及一种与环磷腺苷结合的蛋白质。环磷腺苷是在不添加诱导子实体形成物质就能形成单核子实体的突变菌株的菌丝体内由腺嘌呤合成而来的，但在不能形成单核子实体的菌株中则不能合成。与环磷腺苷结合的蛋白质在能形成单核子实体菌株的菌丝体粗提物以及双核体中被检测到，但在不能形成单核子实体菌株中未被检测到。
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