二丁酰环磷腺苷、糖转运以及正常细胞和转化细胞中细胞分裂的调控
Dibutyryl cyclic adenosine 3'5' monophosphate, sugar transport, and regulatory control of cell division in normal and transformed cells.
作者信息
Grimes W J, Schroeder J L
出版信息
J Cell Biol. 1973 Feb;56(2):487-91. doi: 10.1083/jcb.56.2.487.
Abstract
Swiss 3T3 cells exhibit contact-regulated cell growth and have a lower ability to transport 2-deoxyglucose than polyoma (Py)-transformed 3T3 cells. Py3T3 cells treated with dibutyryl cyclic adenosine 3'5' monophosphate (dBcAMP) and theophylline have reduced cell growth and transport 2-deoxyglucose at the same rate as normal 3T3 cells. Evidence that the cessation of cell growth and reduced transport abilities in Py3T3 cells does not represent a return to contact-regulated growth comes from the following observations. First, treating high density Py3T3 cells with dBcAMP allows more than two doublings of cell number, even though ability to transport 2-deoxyglucose is returned to levels equal to those of normal 3T3 cells. Second, dBcAMP prevents serum-stimulated increases in 2-deoxyglucose transport in Py3T3 but not in 3T3 cells.
摘要
瑞士3T3细胞表现出接触调节的细胞生长，并且与多瘤病毒（Py）转化的3T3细胞相比，其转运2-脱氧葡萄糖的能力较低。用二丁酰环磷腺苷（dBcAMP）和茶碱处理的Py3T3细胞生长减缓，并且转运2-脱氧葡萄糖的速率与正常3T3细胞相同。Py3T3细胞中细胞生长停止和转运能力降低并不代表恢复到接触调节生长，以下观察结果提供了证据。首先，用dBcAMP处理高密度Py3T3细胞可使细胞数量增加两倍以上，尽管转运2-脱氧葡萄糖的能力恢复到与正常3T3细胞相同的水平。其次，dBcAMP可阻止血清刺激的Py3T3细胞中2-脱氧葡萄糖转运增加，但对3T3细胞没有此作用。
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