Lam K M, Hsiung G D
Infect Immun. 1973 Mar;7(3):426-31. doi: 10.1128/iai.7.3.426-431.1973.
Guinea pigs, experimentally infected with guinea pig herpes-like virus produced antibodies which were detectable by indirect hemagglutination (IHA), complement-fixation (CF), and neutralization tests. The IHA test appeared to be a more sensitive method than the CF or neutralization test for determining antibody response in guinea pigs immediately after infection, but the IHA method was not suitable for the detection of antibody in animals receiving small doses of virus. Antibody titers obtained by CF tests were generally higher than those obtained by the neutralization test, and they followed the same time course when individual animals were studied serially. Intracardiac inoculation produced the best antibody response in guinea pigs when compared with other routes of infection. Guinea pigs infected by the intraperitoneal, intranasal, or oral route showed rising antibody titers but the levels were low. Infectious virus was isolated from and persisted in all inoculated animals in the presence of antibody regardless of the route of inoculation. Recovery of infectious virus required cultivation or cocultivation of tissue cells containing virus. The administration of antilymphocyte sera delayed the appearance of IHA antibody but had no effect on antibodies determined by the CF- and neutralization tests.
用豚鼠疱疹样病毒实验感染豚鼠后，所产生的抗体可用间接血凝试验（IHA）、补体结合试验（CF）和中和试验检测出来。在感染后立即测定豚鼠的抗体反应时，IHA试验似乎比CF试验或中和试验更为敏感的方法，但IHA方法不适用于检测接受小剂量病毒的动物体内的抗体。通过CF试验获得的抗体效价一般高于通过中和试验获得的抗体效价，当对个体动物进行连续研究时，它们遵循相同的时间进程。与其他感染途径相比，心脏内接种在豚鼠中产生的抗体反应最佳。经腹腔、鼻内或口服途径感染的豚鼠抗体效价升高，但水平较低。无论接种途径如何，在有抗体存在的情况下，均能从所有接种动物中分离出传染性病毒并持续存在。传染性病毒的回收需要对含有病毒的组织细胞进行培养或共培养。抗淋巴细胞血清的给予延迟了IHA抗体的出现，但对CF试验和中和试验所测定的抗体没有影响。
