Hilfrich J
Br J Cancer. 1973 Jul;28(1):46-54. doi: 10.1038/bjc.1973.69.
After the implantation of ovarian tissue into the spleen of gonadectomized female Sprague-Dawley rats (splenic ovary), luteomata and later benign granulosa or granulosa-theca cell tumours develop. Treatment of these rats with 7,12 dimethylbenz(a)anthracene (DMBA), given intravenously, 2 mg/kg body weight weekly, total dosage 40 mg/kg, immediately and especially 25 weeks after implantation of ovarian tissue into the spleen, led to malignant, partially metastasizing granulosa, and in one case theca cell tumours, 16-46 weeks after beginning the carcinogen treatment. No malignant neoplastic growth was seen when diethylnitrosamine (DEN), 20 mg/kg once weekly for life, was injected subcutaneously immediately or 25 weeks after implanting ovarian tissue.Since the normal, non-implanted rat ovary was not affected by DMBA treatment the malignant transformation of splenic ovaries in the respective experimental groups may be related to the increased stimulation by pituitary gonadotrophins and formation of luteomata or beginning granulosa and theca cell proliferations.
将卵巢组织植入去性腺的雌性斯普拉格-道利大鼠的脾脏（脾内卵巢）后，会出现黄体瘤，随后发展为良性颗粒细胞瘤或颗粒-卵泡膜细胞瘤。对这些大鼠静脉注射7,12-二甲基苯并(a)蒽（DMBA），每周2毫克/千克体重，总剂量40毫克/千克，在将卵巢组织植入脾脏后立即进行，尤其是在植入后25周进行，在开始致癌剂治疗后16至46周，导致恶性的、部分转移的颗粒细胞瘤，在一例中为卵泡膜细胞瘤。当每周皮下注射一次二乙基亚硝胺（DEN），剂量为20毫克/千克，持续终生，在植入卵巢组织后立即或25周后进行时，未观察到恶性肿瘤生长。由于正常的、未植入的大鼠卵巢不受DMBA治疗的影响，因此各实验组中脾内卵巢的恶性转化可能与垂体促性腺激素的刺激增加以及黄体瘤的形成或颗粒细胞和卵泡膜细胞的增殖开始有关。
