前列腺素脱氢酶抑制剂对大鼠离体肺中前列腺素摄取的作用。
Action of prostaglandin dehydrogenase inhibitors on prostaglandin uptake in rat isolated lung.
作者信息
Bakhle Y S
出版信息
Br J Pharmacol. 1979 Apr;65(4):635-9. doi: 10.1111/j.1476-5381.1979.tb07875.x.
Abstract
- The effects of some inhibitors of 15-hydroxy-prostaglandin dehydrogenase (PGDH) have been studied on the inactivation of prostaglandin E2 (PGE2) and 16, 16-dimethyl PGE2 in rat isolated lung. 2. Bioassay was used to show that the inactivation of PGE2 and its methyl analogue, was inhibited by frusemide (10(-3) M), N-ethyl maleimide (10(-5) M) and 5,5'-dithio-bis-2-nitro-benzoic acid (10(-2) M), but not by caffeine (2.5 x 10(-3) M). 3. The efflux of radioactivity from lung following injection of [14C]-PGE2 was faster in lungs treated with frusemide, N-ethyl maleimide or bromcresol green, than in untreated lungs. 4. Caffeine (10(-3), 10(-2) M) did not change the rate of 14C-efflux from lungs following injection of [14C]-PGE2. 5. From these results it is concluded that those PGDH inhibitors that prevented prostaglandin inactivation in isolated lungs did so by inhibiting uptake of prostaglandin rather than by inhibiting PGDH.
摘要
- 研究了15-羟基前列腺素脱氢酶（PGDH）的某些抑制剂对大鼠离体肺中前列腺素E2（PGE2）和16,16-二甲基PGE2失活的影响。2. 生物测定表明，速尿（10⁻³ M）、N-乙基马来酰亚胺（10⁻⁵ M）和5,5'-二硫代双-2-硝基苯甲酸（10⁻² M）可抑制PGE2及其甲基类似物的失活，但咖啡因（2.5×10⁻³ M）则无此作用。3. 注射[¹⁴C]-PGE2后，用速尿 N-乙基马来酰亚胺或溴甲酚绿处理的肺中放射性物质的流出速度比未处理的肺快。4. 咖啡因（10⁻³、10⁻² M）不会改变注射[¹⁴C]-PGE2后肺中¹⁴C流出的速度。5. 从这些结果可以得出结论，那些能防止离体肺中前列腺素失活的PGDH抑制剂是通过抑制前列腺素的摄取而不是通过抑制PGDH来实现的。
相似文献
1
Action of prostaglandin dehydrogenase inhibitors on prostaglandin uptake in rat isolated lung.前列腺素脱氢酶抑制剂对大鼠离体肺中前列腺素摄取的作用。
Br J Pharmacol. 1979 Apr;65(4):635-9. doi: 10.1111/j.1476-5381.1979.tb07875.x.
2
Pulmonary uptake of PGE2 is inhibited by dipyridamole in rat isolated lungs.在大鼠离体肺中，双嘧达莫可抑制前列腺素E2的肺摄取。
Prostaglandins. 1982 Feb;23(2):147-56. doi: 10.1016/0090-6980(82)90042-9.
3
Inhibitors of prostaglandin dehydrogenase (Ph CL 28A and Ph CK 61A) increase output of prostaglandins from rat isolated lung.前列腺素脱氢酶抑制剂（Ph CL 28A和Ph CK 61A）可增加大鼠离体肺中前列腺素的输出量。
Br J Pharmacol. 1987 Sep;92(1):189-96. doi: 10.1111/j.1476-5381.1987.tb11311.x.
4
Effects of sulphasalazine on prostaglandin inactivation and synthesis in isolated lungs of guinea pig, rat and man.柳氮磺胺吡啶对豚鼠、大鼠和人离体肺中前列腺素失活及合成的影响。
Eur J Pharmacol. 1980 Dec 19;68(4):493-6. doi: 10.1016/0014-2999(80)90425-2.
5
The metabolism of prostaglandin E2 in perinatal rabbit lungs.
Prostaglandins. 1983 Aug;26(2):275-85. doi: 10.1016/0090-6980(83)90095-3.
6
The metabolism of prostaglandin E2 is decreased by sulfinpyrazone in isolated hamster lungs.
Prostaglandins Med. 1981 Apr;6(4):441-7. doi: 10.1016/0161-4630(81)90077-x.
7
Highly potent inhibition of prostaglandin 15-hydroxydehydrogenase in-vitro and of prostaglandin inactivation in perfused lung by the new azobenzene analogue, Ph CL 28A.
J Pharm Pharmacol. 1985 Sep;37(9):622-8. doi: 10.1111/j.2042-7158.1985.tb05098.x.
8
Inactivation of prostaglandin E2 by rat isolated lung during the oestrous cycle.大鼠离体肺在发情周期中对前列腺素E2的灭活作用。
J Endocrinol. 1983 Jul;98(1):47-53. doi: 10.1677/joe.0.0980047.
9
Identification of 6-oxo-prostaglandin E1 as a naturally occurring prostanoid generated by rat lung.鉴定6-氧代前列腺素E1为大鼠肺产生的一种天然存在的类前列腺素。
Br J Pharmacol. 1986 Feb;87(2):327-35. doi: 10.1111/j.1476-5381.1986.tb10821.x.
10
Enhanced thromboxane B2 release from challenged guinea pig lung after oxygen exposure.氧气暴露后，受刺激的豚鼠肺中血栓素B2释放增强。
Am Rev Respir Dis. 1980 Apr;121(4):695-9. doi: 10.1164/arrd.1980.121.4.695.
引用本文的文献
1
Inhibition by clinically used dyes of prostaglandin inactivation in rat and human lung.临床使用的染料对大鼠和人肺中前列腺素失活的抑制作用。
Br J Pharmacol. 1981 Apr;72(4):715-21. doi: 10.1111/j.1476-5381.1981.tb09153.x.
2
Effect of sulphasalazine on pulmonary inactivation of prostaglandin F2 alpha in the pig.柳氮磺胺吡啶对猪体内前列腺素F2α肺灭活作用的影响。
Br J Pharmacol. 1982 Jun;76(2):319-26. doi: 10.1111/j.1476-5381.1982.tb09223.x.
3
Effect of nafazatrom and indomethacin on pulmonary removal of prostaglandin E1 after endotoxin in rabbits.萘呋胺酯和吲哚美辛对兔内毒素血症后肺清除前列腺素E1的影响。
Br J Pharmacol. 1987 Aug;91(4):721-8. doi: 10.1111/j.1476-5381.1987.tb11269.x.
4
Inhibitors of prostaglandin dehydrogenase (Ph CL 28A and Ph CK 61A) increase output of prostaglandins from rat isolated lung.前列腺素脱氢酶抑制剂（Ph CL 28A和Ph CK 61A）可增加大鼠离体肺中前列腺素的输出量。
Br J Pharmacol. 1987 Sep;92(1):189-96. doi: 10.1111/j.1476-5381.1987.tb11311.x.
本文引用的文献
1
A sensitive method for the assay of 5-hydroxytryptamine.一种测定5-羟色胺的灵敏方法。
Br J Pharmacol Chemother. 1957 Sep;12(3):344-9. doi: 10.1111/j.1476-5381.1957.tb00146.x.
2
Removal of 5-hydroxytryptamine in the pulmonary circulation of rat isolated lungs.大鼠离体肺脏肺循环中5-羟色胺的清除
Br J Pharmacol. 1970 Nov;40(3):468-82.
3
Preparative thin-layer and column chromatography of prostaglandins.前列腺素的制备型薄层色谱法和柱色谱法。
J Lipid Res. 1969 May;10(3):316-9.
4
The use of the hamster stomach in vitro as an assay preparation for prostaglandins.将仓鼠胃用于体外作为前列腺素的一种检测制剂。
Br J Pharmacol. 1973 Dec;49(4):662-6. doi: 10.1111/j.1476-5381.1973.tb08542.x.
5
Prostaglandin inactivation in guinea-pig lung and its inhibition.豚鼠肺中前列腺素的失活及其抑制作用。
Br J Pharmacol. 1974 Oct;52(2):197-203. doi: 10.1111/j.1476-5381.1974.tb09700.x.
6
Inhibition of pulmonary prostaglandin metabolism by inhibitors of prostaglandin biotransport (probenecid and bromcresol green).通过前列腺素生物转运抑制剂（丙磺舒和溴甲酚绿）抑制肺部前列腺素代谢。
Prostaglandins. 1975 Oct;10(4):633-9. doi: 10.1016/s0090-6980(75)80010-4.
7
Colorimetric identification of prostaglandins in subnanomole amounts.
Anal Biochem. 1975 Sep;68(1):336-40. doi: 10.1016/0003-2697(75)90714-9.
8
Inhibition of the pulmonary inactivation of prostaglandins in vivo by di-4-phloretin phosphate.磷酸二对香豆素对体内前列腺素肺失活的抑制作用。
Br J Pharmacol. 1975 Jul;54(3):301-7. doi: 10.1111/j.1476-5381.1975.tb07569.x.
9
Prostaglandin removal and metabolism by isolated perfused rat lung.前列腺素在离体灌注大鼠肺中的清除与代谢
Prostaglandins. 1976 Apr;11(4):645-77. doi: 10.1016/0090-6980(76)90067-8.
10
Uptake and inactivation of prostaglandin E2 methyl analogues in the rat pulmonary circulation.前列腺素E2甲基类似物在大鼠肺循环中的摄取与失活
Br J Pharmacol. 1978 Feb;62(2):275-80. doi: 10.1111/j.1476-5381.1978.tb08456.x.
Zotero 插件