将仓鼠胃用于体外作为前列腺素的一种检测制剂。
The use of the hamster stomach in vitro as an assay preparation for prostaglandins.
作者信息
Ubatuba F B
出版信息
Br J Pharmacol. 1973 Dec;49(4):662-6. doi: 10.1111/j.1476-5381.1973.tb08542.x.
Abstract
- Hamster stomach strips in vitro are useful test preparations for the assay of prostaglandins E and F in the nanogram concentration range. Three-minute cycles can be used for long periods without spontaneous contractions or significant base-line instability.2. The mean percentage error and the precision index of six 4-point assays of prostaglandin E(2) were 7.8 and 0.057, respectively.3. The relative sensitivity to different prostaglandins was E(2)/E(1) approximately 1, E(2)/F(1alpha) approximately 65 and E(2)/A(1) approximately 400.4. These preparations were relatively insensitive to 5-hydroxytryptamine (prostaglandin E(2)/5-HT approximately 20,000) and histamine (prostaglandin E(2)/histamine approximately 200).5. These results suggest that the hamster stomach preparation can be used to differentiate between 5-hydroxytryptamine and prostaglandins.
摘要
体外仓鼠胃条是用于测定纳克浓度范围内前列腺素E和F的有用试验制剂。三分钟周期可长时间使用，不会出现自发收缩或明显的基线不稳定。
前列腺素E(2)的六项4点测定的平均百分比误差和精密度指数分别为7.8和0.057。
对不同前列腺素的相对敏感性为E(2)/E(1)约为1，E(2)/F(1α)约为65，E(2)/A(1)约为400。
这些制剂对5-羟色胺（前列腺素E(2)/5-HT约为20,000）和组胺（前列腺素E(2)/组胺约为200）相对不敏感。
这些结果表明，仓鼠胃制剂可用于区分5-羟色胺和前列腺素。
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