Sipe J C, Herman M M, Rubinstein L J
Am J Pathol. 1973 Dec;73(3):589-606.
The fine structure of four glioblastomas and two cerebellar astrocytomas maintained in organ culture systems up to 137 days and 43 days, respectively, using either a three-dimensional sponge foam matrix technic or a Millipore filter platform technic, is described and compared. The cells of both tumor types showed increased astrocytic differentiation, characterized by a progressive increase in glial filaments associated with an increase in free ribosomes and granular endoplasmic reticulum. A progressive increase in basement membrane material, presumably originating from explanted endothelial cells or pericytes, was also found in both tumor types and was often associated with increased numbers of collagen fibrils. Astrocytic tumor cell processes frequently preserved their contact with this basement membrane material. Microvascular fenestrations or gaps in endothelial cells were not identified. These electron microscopic features appear to correspond to the early stages of perivascular sclerosis previously noted by light microscopy in gliomas maintained in organ culture systems and are presumably related to the progressive obliteration of the functional microvasculature.
描述并比较了分别使用三维海绵泡沫基质技术或微孔滤膜平台技术在器官培养系统中维持长达137天和43天的4例胶质母细胞瘤和2例小脑星形细胞瘤的精细结构。两种肿瘤类型的细胞均显示星形细胞分化增加，其特征是胶质细丝逐渐增加，同时游离核糖体和粗面内质网也增加。在两种肿瘤类型中还发现基底膜物质逐渐增加，推测其起源于植入的内皮细胞或周细胞，并且通常与胶原纤维数量增加有关。星形细胞瘤细胞突起经常保持与这种基底膜物质的接触。未发现微血管窗孔或内皮细胞间隙。这些电子显微镜特征似乎与之前在器官培养系统中维持的胶质瘤光镜下观察到的血管周围硬化早期阶段相对应，并且可能与功能性微血管的逐渐闭塞有关。
