噬菌体T4的chi突变的生化研究：丝裂霉素C对chi+和chi DNA合成的差异抑制
Biochemical studies on the chi mutation of bacteriophage T4: differential inhibition of chi+ and chi DNA synthesis by mitomycin C.
作者信息
Shimizu K, Sekiguchi M
出版信息
J Virol. 1974 Jan;13(1):1-8. doi: 10.1128/JVI.13.1.1-8.1974.
Abstract
Biochemical studies were carried out to determine the effect of chi mutation on T4 DNA synthesis. The rate and final extent of DNA synthesis are almost the same with T4D- and T4chi-infected cells, although the burst size of T4chi is about one-sixth that of the wild type. The DNA synthesis of T4chi-infected cells is more readily inhibited by mitomycin C than is that of T4 wild type. When mitomycin C was added during active phage growth, DNA synthesis of T4chi halted almost immediately. T4 DNA polymerases isolated from chi(+)- and chi-infected cells, however, exhibit no difference with regard to their sensitivities to mitomycin C, priming activities with alkylated or ultraviolet light-irradiated templates and other enzymatic properties.
摘要
进行了生化研究以确定chi突变对T4 DNA合成的影响。T4D感染的细胞和T4chi感染的细胞中DNA合成的速率和最终程度几乎相同，尽管T4chi的爆发量约为野生型的六分之一。与T4野生型相比，丝裂霉素C对T4chi感染细胞的DNA合成抑制作用更强。当在噬菌体活跃生长期间添加丝裂霉素C时，T4chi的DNA合成几乎立即停止。然而，从chi(+)感染细胞和chi感染细胞中分离出的T4 DNA聚合酶，在对丝裂霉素C的敏感性、对烷基化或紫外线照射模板的引发活性以及其他酶学性质方面没有差异。
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