一种具有温度敏感性亮氨酰转运RNA合成酶的哺乳动物细胞突变体。
A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase.
作者信息
Thompson L H, Harkins J L, Stanners C P
出版信息
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3094-8. doi: 10.1073/pnas.70.11.3094.
Abstract
A cell mutant of the Chinese hamster ovary line, which is temperature sensitive for protein synthesis, is specifically defective in vivo in its ability to charge tRNA with leucine. Cytoplasmic extracts exhibited temperature-sensitive leucyl-tRNA synthetase activity. It is, therefore, highly likely that the mutant has a structural alteration in leucyl-tRNA synthetase. The low leakiness and low reversion rate of this mutant, combined with the specificity of the defect in its protein-synthesizing machinery, make it an appealing tool for investigating regulatory mechanisms in animal cells.
摘要
中国仓鼠卵巢细胞系的一个细胞突变体，其对蛋白质合成具有温度敏感性，在体内将亮氨酸加载到tRNA上的能力存在特异性缺陷。细胞质提取物表现出温度敏感的亮氨酰-tRNA合成酶活性。因此，该突变体极有可能在亮氨酰-tRNA合成酶上存在结构改变。该突变体的低渗漏率和低回复率，再加上其蛋白质合成机制缺陷的特异性，使其成为研究动物细胞调控机制的一个有吸引力的工具。
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本文引用的文献
1
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J Biol Chem. 1951 Nov;193(1):265-75.
2
Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.体细胞哺乳动物细胞遗传学。III. 来自人类和动物受试者的整倍体细胞的长期培养。
J Exp Med. 1958 Dec 1;108(6):945-56. doi: 10.1084/jem.108.6.945.
3
Plaque formation and isolation of pure lines with poliomyelitis viruses.脊髓灰质炎病毒的噬斑形成及纯系分离
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.
4
Polyribosomes of hamster cells: transit time measurements.仓鼠细胞的多核糖体：转运时间测量
Biophys J. 1968 Feb;8(2):231-51. doi: 10.1016/S0006-3495(68)86487-2.
5
Control of macromolecular synthesis in proliferating and resting Syrian hamster cells in monolayer culture. I. Ribosome function.单层培养的增殖和静止叙利亚仓鼠细胞中大分子合成的调控。I. 核糖体功能。
J Cell Physiol. 1971 Feb;77(1):31-42. doi: 10.1002/jcp.1040770105.
6
Two types of ribosome in mouse-hamster hybrid cells.小鼠-仓鼠杂交细胞中的两种核糖体。
Nat New Biol. 1971 Mar 10;230(10):52-4. doi: 10.1038/newbio230052a0.
7
A role of aminoacyl-tRNA in the regulation of protein breakdown in Escherichia coli.氨酰tRNA在大肠杆菌蛋白质降解调控中的作用。
Proc Natl Acad Sci U S A. 1971 Feb;68(2):362-6. doi: 10.1073/pnas.68.2.362.
8
Transfer RNA species in normal and leukemic human lymphoblasts.正常和白血病人类淋巴母细胞中的转运RNA种类
J Mol Biol. 1970 Sep 14;52(2):195-219. doi: 10.1016/0022-2836(70)90025-2.
9
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Proc Natl Acad Sci U S A. 1970 Jun;66(2):377-84. doi: 10.1073/pnas.66.2.377.
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Mutation rates in cells at different ploidy levels.不同倍性水平细胞中的突变率。
J Cell Physiol. 1971 Oct;78(2):177-84. doi: 10.1002/jcp.1040780204.
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