仓鼠细胞的多核糖体：转运时间测量
Polyribosomes of hamster cells: transit time measurements.
作者信息
Stanners C P
出版信息
Biophys J. 1968 Feb;8(2):231-51. doi: 10.1016/S0006-3495(68)86487-2.
Abstract
The amount of radioactivity incorporated into completed polypeptides and into nascent polypeptides bound to polyribosomes was measured for rapidly dividing, cultured hamster embryo cells incubated in the presence of labelled amino acids. Using a mathematical analysis which took into account variations in the specific activity of the intracellular amino acid pool, these measurements yielded a value for the length of time required for the synthesis of a primary polypeptide of average length, the "transit time." Subject to a number of reasonable assumptions in the mathematical analysis which were, however, only approximately verified, the transit time was found to be 23 +/- 2 sec in four independent experiments. Regardless of the absolute accuracy of this measurement, the method is considered a useful measure of translation independent of transcription.
摘要
对于在标记氨基酸存在下培养的快速分裂的仓鼠胚胎细胞，测量了掺入完整多肽和与多核糖体结合的新生多肽中的放射性量。通过考虑细胞内氨基酸池比活度变化的数学分析，这些测量得出了合成平均长度的初级多肽所需的时间值，即“转运时间”。尽管在数学分析中有一些合理假设，但仅得到了大致验证，在四个独立实验中发现转运时间为23±2秒。无论该测量的绝对准确性如何，该方法都被认为是一种独立于转录的有用的翻译测量方法。
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Proc Natl Acad Sci U S A. 1963 May;49(5):654-62. doi: 10.1073/pnas.49.5.654.
2
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Proc Natl Acad Sci U S A. 1960 Mar;46(3):365-70. doi: 10.1073/pnas.46.3.365.
3
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Biochem Biophys Res Commun. 1965 Feb 3;18:389-95. doi: 10.1016/0006-291x(65)90719-9.
4
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J Mol Biol. 1964 Jul;9:213-35. doi: 10.1016/s0022-2836(64)80102-9.
5
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6
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7
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Virology. 1963 Nov;21:383-9. doi: 10.1016/0042-6822(63)90199-5.
8
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9
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10
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