冷保存豚鼠盲肠对（3H）-腺苷核苷酸和（3H）-去甲肾上腺素的摄取；去甲肾上腺素、罂粟碱和硝酸甘油对（3H）-腺苷核苷酸的机械作用及释放

(3H)-adenosine nucleotide and (3H)-noradrenaline uptake by cold stored guinea-pig taenia caecum; mechanical effects and release of (3H)-adenosine nucleotide by noradrenaline, papaverine, and nitroglycerine.

作者信息

Kuchii M, Miyahara J T, Shibata S

出版信息

Br J Pharmacol. 1973 Dec;49(4):642-50. doi: 10.1111/j.1476-5381.1973.tb08539.x.

DOI:10.1111/j.1476-5381.1973.tb08539.x

PMID:4362810

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1776604/

Abstract

Cold storage (2 degrees C) treatment progressively reduced noradrenaline uptake by the taenia caecum of the guinea-pig. After 15 days of cold treatment, [(3)H]-noradrenaline uptake by tissue was reduced to about 10% of control. On the other hand, prolonged cold storage failed to decrease [(3)H]-adenosine uptake by the taenia caecum.2. Cocaine (10 muM) inhibited noradrenaline uptake by about 82% but nucleoside uptake was inhibited by 20%. Cocaine treatment failed to decrease the residual noradrenaline uptake in the cold stored strips (more than 10 days). Phenoxybenzamine (1 muM) or oligomycin (1 mug/ml) treatment decreased the tissue adenosine uptake to about 34% and 28% of the respective controls.3. Based on thin layer chromatography, it was estimated that approximately 68% of [(3)H]-adenosine was converted into and retained as [(3)H]-ATP in the fresh tissues, a small fraction was accountable as [(3)H]-adenosine (18%) but virtually no [(3)H]-AMP, [(3)H]-cyclic AMP or [(3)H]-ADP was detected. Similar distribution of radioactivity of nucleotides was observed in tissues cold stored for 8 days.4. The inhibition of the mechanical activity of taenia by noradrenaline (10 muM), papaverine (100 muM) and nitroglycerine (100 muM) was accompanied by [(3)H]-adenine nucleotide but not [(3)H]-noradrenaline release. Treatment with phentolamine and propranolol (both 1 muM) had no effect on the adenine nucleotide release elicited by nicotine and electrical field stimulation, whereas such treatment reduced the inhibitory action of both stimuli.5. These results suggest that the nucleotide release after application of electrical and chemical stimulation may be from an extraneurogenic source. Thus, we conclude that ATP or a related nucleotide is not the chemical transmitter of the non-adrenergic inhibition in the taenia caecum of the guinea-pig.

摘要

冷藏（2摄氏度）处理会逐渐降低豚鼠盲肠带对去甲肾上腺素的摄取。冷藏15天后，组织对[³H] - 去甲肾上腺素的摄取降至对照的约10%。另一方面，长时间冷藏未能降低盲肠带对[³H] - 腺苷的摄取。
可卡因（10 μM）抑制去甲肾上腺素摄取约82%，但核苷摄取受抑制20%。可卡因处理未能降低冷藏超过10天的条带中残余的去甲肾上腺素摄取。酚苄明（1 μM）或寡霉素（1 μg/ml）处理使组织腺苷摄取分别降至各自对照的约34%和28%。
根据薄层色谱法估计，新鲜组织中约68%的[³H] - 腺苷转化并保留为[³H] - ATP，一小部分为[³H] - 腺苷（18%），但几乎未检测到[³H] - AMP、[³H] - 环磷酸腺苷或[³H] - ADP。在冷藏8天的组织中观察到核苷酸放射性的类似分布。
去甲肾上腺素（10 μM）、罂粟碱（100 μM）和硝酸甘油（100 μM）对盲肠带机械活性的抑制伴随着[³H] - 腺嘌呤核苷酸而非[³H] - 去甲肾上腺素的释放。酚妥拉明和普萘洛尔（均为1 μM）处理对尼古丁和电场刺激引起的腺嘌呤核苷酸释放无影响，而这种处理降低了两种刺激的抑制作用。
这些结果表明，电刺激和化学刺激后核苷酸的释放可能来自神经外源性来源。因此，我们得出结论，ATP或相关核苷酸不是豚鼠盲肠带非肾上腺素能抑制的化学递质。