Shibata S, Miyahara J T, Mori J, Ohashi T
Eur J Pharmacol. 1977 May 1;43(1):47-56. doi: 10.1016/0014-2999(77)90159-5.
After cold storage of guinea-pig taenia strips for 4 days at 2 degrees C, structures with specific catecholamine fluorescence were not observed. Upon incubation in Krebs Ringer medium (37 degrees C) the fluorescence in the cold stored tissue reappeared and the density of fluorescence was comparable to that of fresh preparations. The specific fluorescence was affected following reserpine or 6-hydroxydopamine treatment; however, the rewarming process restored fluorescence only in the reserpine-treated tissue. The cold storage treatment (4 and 7 days) also decreased the tissue noradrenaline and dopamine contents and dopamine-beta-hydroxylase activity and inhibited the [3H-noradrenaline release from the tissue, all of which were not restored by the incubation procedure. Moreover, in these cold stored taenia the inhibitory effect to nicotine and perivascular nerve stimulation were not manifested. Therefore, the reappearance of specific fluorescence in the cold stored taenia strips after rewarming does not assure functional recovery of adrenergic nerve activity.
将豚鼠绦虫条在2℃下冷藏4天后，未观察到具有特定儿茶酚胺荧光的结构。在 Krebs-Ringer 培养基（37℃）中孵育后，冷藏组织中的荧光重新出现，荧光密度与新鲜制剂相当。利血平或6-羟基多巴胺处理后，特异性荧光受到影响；然而，复温过程仅使利血平处理的组织中的荧光得以恢复。冷藏处理（4天和7天）还降低了组织去甲肾上腺素和多巴胺含量以及多巴胺-β-羟化酶活性，并抑制了[3H]-去甲肾上腺素从组织中的释放，所有这些均未通过孵育程序恢复。此外，在这些冷藏的绦虫中，对尼古丁和血管周围神经刺激的抑制作用未表现出来。因此，冷藏的绦虫条复温后特异性荧光的重新出现并不能确保肾上腺素能神经活动的功能恢复。
