Kuchii M, Miyahara J T, Shibata S
Br J Pharmacol. 1974 Aug;51(4):577-83. doi: 10.1111/j.1476-5381.1974.tb09677.x.
1 The effects of pretreatment of guinea-pigs with 6-hydroxydopamine (6-OHDA) on the release of [(3)H]-noradrenaline and [(3)H]-adenine nucleotide following electrical field, nicotine and perivascular nerve stimulation of guinea-pig taenia caecum were studied.2 High frequency electrical field stimulation (15,30 Hz) released both [(3)H]-noradrenaline and [(3)H]-adenine nucleotide but low frequency (0.5, 5 Hz) stimulation, producing comparable muscle relaxation led only to [(3)H]-noradrenaline release.3 6-OHDA (50-200 mg/kg) pretreatment inhibited the muscle relaxation and [(3)H]-noradrenaline release with electrical stimulation or with nicotine in isolated taenia but did not affect the release of [(3)H]-nucleotide.4 A low dose of 6-OHDA (50 mg/kg), completely inhibited the muscle relaxation and [(3)H]-noradrenaline release elicited by perivascular nerve stimulation.5 Both tissue noradrenaline content and [(3)H]-noradrenaline uptake were decreased to the same extent by low as well as high doses of 6-OHDA: noradrenaline content was reduced to 20% and uptake to 30% of the control value.6 Catecholamine fluorescence disappeared from tissue layers of the taenia after treatment with a high dose of 6-OHDA.7 In these experiments the inhibitory action of electrical stimulation and nicotine on the taenia can be correlated better with noradrenaline than with nucleotide release.
1 研究了用6-羟基多巴胺（6-OHDA）预处理豚鼠后，电场、尼古丁和对豚鼠盲肠带进行血管周围神经刺激对[（3）H]-去甲肾上腺素和[（3）H]-腺嘌呤核苷酸释放的影响。
2 高频电场刺激（15、30Hz）可释放[（3）H]-去甲肾上腺素和[（3）H]-腺嘌呤核苷酸，但低频（0.5、5Hz）刺激在产生相当的肌肉松弛时仅导致[（3）H]-去甲肾上腺素释放。
3 6-OHDA（50-200mg/kg）预处理可抑制离体盲肠带经电刺激或尼古丁刺激后的肌肉松弛和[（3）H]-去甲肾上腺素释放，但不影响[（3）H]-核苷酸的释放。
4 低剂量的6-OHDA（50mg/kg）可完全抑制血管周围神经刺激引起的肌肉松弛和[（3）H]-去甲肾上腺素释放。
5 低剂量和高剂量的6-OHDA均可使组织去甲肾上腺素含量和[（3）H]-去甲肾上腺素摄取量降低到相同程度：去甲肾上腺素含量降至对照值的20%，摄取量降至30%。
6 用高剂量的6-OHDA处理后，盲肠带组织层的儿茶酚胺荧光消失。
7 在这些实验中，电刺激和尼古丁对盲肠带的抑制作用与去甲肾上腺素释放的相关性比对核苷酸释放的相关性更好。
