电场刺激、血管周围神经刺激以及尼古丁诱发的豚鼠盲肠带绦虫释放（3H）-腺嘌呤核苷酸和（3H）-去甲肾上腺素。

(3H)-adenine nucleotide and (3H)-noradrenaline release evoked by electrical field stimulation, perivascular nerve stimulation and nicotine from the taenia of the guinea-pig caecum.

Kuchii M, Miyahara J T, Shibata S

Br J Pharmacol. 1973 Oct;49(2):258-66. doi: 10.1111/j.1476-5381.1973.tb08371.x.

Abstract

The release of [(3)H]-noradrenaline and adenine nucleotide evoked by electrical field stimulation (20-60 V, 30 Hz), perivascular nerve stimulation (20-80 V, 60 Hz) and nicotine (10, 100 muM) was studied in the taenia of the guinea-pig caecum under various conditions.2. Electrical stimulation at high intensity (60 V) caused the release of [(3)H]-adenine nucleotide; however, the inhibitory action of electrical stimulation was proportional to [(3)H]-noradrenaline release.3. The intensity of the inhibitory effect of stimulation of the perivascular nerves was directly related to [(3)H]-noradrenaline release and not associated with the release of [(3)H]-nucleotide.4. Cold storage for more than 8 days, cooling (19 degrees C) or tetrodotoxin treatment (1 mug/ml) abolished the inhibitory responses to electrical stimulation and to nicotine. After these treatments, nicotine and electrical stimulation elicited only contractions; the release of [(3)H]-noradrenaline, but not that of [(3)H]-adenine nucleotide, was inhibited.5. The dissociation of the inhibitory effects of electrical stimulation and nicotine from [(3)H]-nucleotide release does not support the hypothesis that ATP or a related nucleotide is the humoral transmitter of the non-adrenergic inhibition in the taenia of the guinea-pig caecum.

摘要

在不同条件下，研究了电场刺激（20 - 60 V，30 Hz）、血管周围神经刺激（20 - 80 V，60 Hz）和尼古丁（10、100 μM）诱发豚鼠盲肠带中[（3）H] - 去甲肾上腺素和腺嘌呤核苷酸的释放情况。
高强度（60 V）电刺激导致[（3）H] - 腺嘌呤核苷酸释放；然而，电刺激的抑制作用与[（3）H] - 去甲肾上腺素释放成正比。
血管周围神经刺激的抑制作用强度与[（3）H] - 去甲肾上腺素释放直接相关，与[（3）H] - 核苷酸释放无关。
冷藏超过8天、冷却（19℃）或河豚毒素处理（1 μg/ml）消除了对电刺激和尼古丁的抑制反应。经过这些处理后，尼古丁和电刺激仅引起收缩；[（3）H] - 去甲肾上腺素的释放受到抑制，但[（3）H] - 腺嘌呤核苷酸的释放未受抑制。
电刺激和尼古丁的抑制作用与[（3）H] - 核苷酸释放的解离不支持ATP或相关核苷酸是豚鼠盲肠带非肾上腺素能抑制的体液递质这一假说。