产气荚膜梭菌计数的快速技术
RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS.
作者信息
MARSHALL R S, STEENBERGEN J F, MCCLUNG L S
出版信息
Appl Microbiol. 1965 Jul;13(4):559-63. doi: 10.1128/am.13.4.559-563.1965.
Abstract
A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium. Comparisons were made between sulfite-polymyxin-sulfadiazine (SPS) agar and TSN agar at 37 and 46 C with C. perfringens and other organisms. These studies indicate the quantitative and selective superiority of TSN agar, incubated at 46 C, over SPS agar.
摘要
本文描述了一种新的培养基——胰蛋白胨-亚硫酸盐-新霉素（TSN）琼脂，以及一种用于产气荚膜梭菌计数的培养程序。利用产气荚膜梭菌对新霉素的耐受性、在46℃下的最佳生长以及亚硫酸盐还原特性作为培养基开发的基础。在37℃和46℃下，对产气荚膜梭菌和其他微生物，将亚硫酸盐-多粘菌素-磺胺嘧啶（SPS）琼脂与TSN琼脂进行了比较。这些研究表明，在46℃下培养的TSN琼脂在定量和选择性方面优于SPS琼脂。
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