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1
RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS.产气荚膜梭菌计数的快速技术
Appl Microbiol. 1965 Jul;13(4):559-63. doi: 10.1128/am.13.4.559-563.1965.
2
Comparison of media for the enumeration of Clostridium perfringens.用于产气荚膜梭菌计数的培养基比较。
Appl Microbiol. 1971 May;21(5):922-7. doi: 10.1128/am.21.5.922-927.1971.
3
Recovery of heated Clostridium perfringens type A spores on selective media.在选择性培养基上加热产气荚膜梭菌A型孢子的复苏
Appl Microbiol. 1974 Nov;28(5):793-7. doi: 10.1128/am.28.5.793-797.1974.
4
Evaluation and modifications of media for enumeration of Clostridium perfringens.用于产气荚膜梭菌计数的培养基的评估与改进
Appl Microbiol. 1974 Jan;27(1):78-82. doi: 10.1128/am.27.1.78-82.1974.
5
Improved medium for enumeration of Clostridium perfringens.用于产气荚膜梭菌计数的改良培养基。
Appl Microbiol. 1971 Oct;22(4):688-92. doi: 10.1128/am.22.4.688-692.1971.
6
New quantitative, qualitative, and confirmatory media for rapid analysis of food for Clostridium perfringens.用于快速分析产气荚膜梭菌食品的新型定量、定性和确证培养基。
Appl Microbiol. 1971 Mar;21(3):500-6. doi: 10.1128/am.21.3.500-506.1971.
7
Comparison of sulfite-polymyxin-sulfadiazine medium and tryptose-sulfite-cycloserine medium without egg yolk for recovering Clostridium perfringens.用于产气荚膜梭菌复苏的亚硫酸盐-多粘菌素-磺胺嘧啶培养基与不含蛋黄的胰蛋白胨-亚硫酸盐-环丝氨酸培养基的比较。
Appl Environ Microbiol. 1977 Apr;33(4):986-8. doi: 10.1128/aem.33.4.986-988.1977.
8
Studies on the suitability of sulphite cycloserine agar for the enumeration of clostridium perfringens in food and water.亚硫酸盐环丝氨酸琼脂用于食品和水中产气荚膜梭菌计数适用性的研究。
Zentralbl Bakteriol Orig A. 1973 May;223(4):559-61.
9
[The yield of clostridia on selective and non-selective media for anaerobic organisms shown on pure cultures of different species of clostridia].[不同梭菌属物种纯培养物在厌氧生物选择性和非选择性培养基上梭菌的产量]
Zentralbl Bakteriol Orig. 1969 Dec;212(1):109-14.
10
New medium for rapid screening and enumeration of Clostridium perfringens in foods.用于食品中产气荚膜梭菌快速筛选和计数的新培养基。
Appl Environ Microbiol. 1978 Oct;36(4):567-71. doi: 10.1128/aem.36.4.567-571.1978.

引用本文的文献

1
Development of New Tools to Detect Colistin-Resistance among Enterobacteriaceae Strains.用于检测肠杆菌科菌株中黏菌素耐药性的新工具的开发
Can J Infect Dis Med Microbiol. 2018 Dec 5;2018:3095249. doi: 10.1155/2018/3095249. eCollection 2018.
2
Use of bacterial spores in monitoring water quality and treatment.细菌孢子在水质监测与处理中的应用。
J Water Health. 2018 Aug;16(4):491-500. doi: 10.2166/wh.2018.013.
3
Clostridium perfringens type E virulence traits involved in gut colonization.产气荚膜梭菌E型参与肠道定植的毒力特性。
PLoS One. 2015 Mar 23;10(3):e0121305. doi: 10.1371/journal.pone.0121305. eCollection 2015.
4
Comparative Study of Two Methods for Detection of Clostridium perfringens in Ground Beef.对比研究两种检测碎牛肉中产气荚膜梭菌的方法。
Appl Environ Microbiol. 1977 Mar;33(3):735-7. doi: 10.1128/aem.33.3.735-737.1977.
5
A new selective medium for the culture of clostridia from human faeces.一种用于从人粪便中培养梭菌的新型选择性培养基。
Eur J Clin Microbiol. 1982 Oct;1(5):267-71. doi: 10.1007/BF02019969.
6
Antibotulinal efficacy of sulfur dioxide in meat.二氧化硫在肉类中的抗肉毒杆菌作用
Appl Environ Microbiol. 1980 Jun;39(6):1096-9. doi: 10.1128/aem.39.6.1096-1099.1980.
7
Haloduric anaerobes in the sulfide muds of a saline lagoon.盐沼硫化物泥浆中的嗜盐厌氧菌。
Bull Environ Contam Toxicol. 1974 Sep;12(3):346-54. doi: 10.1007/BF01709131.
8
Recovery of heated Clostridium perfringens type A spores on selective media.在选择性培养基上加热产气荚膜梭菌A型孢子的复苏
Appl Microbiol. 1974 Nov;28(5):793-7. doi: 10.1128/am.28.5.793-797.1974.
9
Evaluation of media, time and temperature of incubation, and method of enumeration of several strains fo Clostridium perfringens spores.产气荚膜梭菌几种菌株孢子的培养基、培养时间和温度以及计数方法的评估。
Appl Microbiol. 1974 Apr;27(4):784-92. doi: 10.1128/am.27.4.784-792.1974.
10
Evaluation and modifications of media for enumeration of Clostridium perfringens.用于产气荚膜梭菌计数的培养基的评估与改进
Appl Microbiol. 1974 Jan;27(1):78-82. doi: 10.1128/am.27.1.78-82.1974.

本文引用的文献

1
Human Food Poisoning Due to Growth of Clostridium perfringens (C. welchii) in Freshly Cooked Chicken: Preliminary Note.新鲜烹制鸡肉中产气荚膜梭菌（韦氏梭菌）生长导致的人类食物中毒：初步报告
J Bacteriol. 1945 Aug;50(2):229-31.
2
CHARACTERISTICS OF CLOSTRIDIUM PERFRINGENS STRAINS ASSOCIATED WITH FOOD AND FOOD-BORNE DISEASE.与食品及食源性疾病相关的产气荚膜梭菌菌株特性
J Bacteriol. 1963 May;85(5):1094-103. doi: 10.1128/jb.85.5.1094-1103.1963.
3
Incidence of Clostridium perfringens in American foods.美国食品中产气荚膜梭菌的发生率。
Appl Microbiol. 1963 Jan;11(1):42-4. doi: 10.1128/am.11.1.42-44.1963.
4
[Sulfite-reduction and sulfite-sensitivity of Clostridia. Taxonomic and practical considerations].[梭菌的亚硫酸盐还原与亚硫酸盐敏感性。分类学及实际考量]
Ann Inst Pasteur Lille. 1961;12:175-81.
5
[Contribution to the study of sodium sulfite media for isolation of Clostridium].[亚硫酸钠培养基用于梭菌分离研究的贡献]
Ann Inst Pasteur Lille. 1961;12:183-93.
6
Quantitation of Clostridium perfringens in foods.食品中产气荚膜梭菌的定量分析。
Appl Microbiol. 1962 May;10(3):193-9. doi: 10.1128/am.10.3.193-199.1962.
7
Rapid destruction of bacteria in commonly used diluents and its elimination.常用稀释剂中细菌的快速破坏及其消除。
Appl Microbiol. 1957 Jan;5(1):21-5. doi: 10.1128/am.5.1.21-25.1957.
8
Rapid isolation of Clostridiums by selective inhibition of aerobic flora. I. Use of sorbic acid and polymyxin B sulfate in a liquid medium.通过选择性抑制需氧菌快速分离梭状芽孢杆菌。I. 在液体培养基中使用山梨酸和硫酸多粘菌素B。
Am J Clin Pathol. 1956 Apr;26(4):418-21. doi: 10.1093/ajcp/26.4_ts.418.
9
A selective plate medium for Cl. welchii.用于产气荚膜梭菌的选择性平板培养基。
J Pathol Bacteriol. 1955 Jul;70(1):105-9. doi: 10.1002/path.1700700110.
10
Clostridium welchii food poisoning.魏氏梭菌食物中毒
J Hyg (Lond). 1953 Mar;51(1):75-101. doi: 10.1017/s0022172400015515.

产气荚膜梭菌计数的快速技术

RAPID TECHNIQUE FOR THE ENUMERATION OF CLOSTRIDIUM PERFINGENS.

作者信息

MARSHALL R S, STEENBERGEN J F, MCCLUNG L S

出版信息

Appl Microbiol. 1965 Jul;13(4):559-63. doi: 10.1128/am.13.4.559-563.1965.

DOI:10.1128/am.13.4.559-563.1965
PMID:14339262
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1058297/
Abstract

A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium. Comparisons were made between sulfite-polymyxin-sulfadiazine (SPS) agar and TSN agar at 37 and 46 C with C. perfringens and other organisms. These studies indicate the quantitative and selective superiority of TSN agar, incubated at 46 C, over SPS agar.

摘要

本文描述了一种新的培养基——胰蛋白胨-亚硫酸盐-新霉素（TSN）琼脂，以及一种用于产气荚膜梭菌计数的培养程序。利用产气荚膜梭菌对新霉素的耐受性、在46℃下的最佳生长以及亚硫酸盐还原特性作为培养基开发的基础。在37℃和46℃下，对产气荚膜梭菌和其他微生物，将亚硫酸盐-多粘菌素-磺胺嘧啶（SPS）琼脂与TSN琼脂进行了比较。这些研究表明，在46℃下培养的TSN琼脂在定量和选择性方面优于SPS琼脂。