在产生性感染细胞中猴病毒40转录的链方向。
Strand orientation of simian virus 40 transcription in productively infected cells.
作者信息
Lindstrom D M, Dulbecco R
出版信息
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1517-20. doi: 10.1073/pnas.69.6.1517.
Abstract
Strand orientation of transcription in BSC-1 cells infected by simian virus 40 (SV40) was investigated by annealing RNAs extracted from infected cells with asymmetric complementary RNA from SV40 DNA synthesized in vitro by RNA polymerase from Escherichia coli. The results suggest that the early viral RNA sequences (those made before replication of viral DNA) and the late RNA sequences (those synthesized concurrently with replication of viral DNA) are transcribed from opposite strands of the SV40 DNA. The RNA synthesized in vitro is of the same polarity as the early in vivo RNA and is complementary to the late in vivo RNA. Knowledge of strand selection in lytic infection is a step towards understanding the regulation of transcription of this viral DNA.
摘要
通过将从感染细胞中提取的RNA与由大肠杆菌RNA聚合酶体外合成的猿猴病毒40（SV40）DNA的不对称互补RNA进行退火，研究了感染SV40的BSC-1细胞中转录的链方向。结果表明，早期病毒RNA序列（在病毒DNA复制之前产生的那些序列）和晚期RNA序列（与病毒DNA复制同时合成的那些序列）是从SV40 DNA的相反链转录而来的。体外合成的RNA与体内早期RNA具有相同的极性，并且与体内晚期RNA互补。了解裂解感染中的链选择是朝着理解这种病毒DNA转录调控迈出的一步。
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