Sobsey M D, Wallis C, Melnick J L
Appl Microbiol. 1974 Aug;28(2):232-8. doi: 10.1128/am.28.2.232-238.1974.
Methods were developed for detecting and concentrating enteric viruses in municipal solid waste landfill leachates. Poliovirus added to a leachate was not readily detectable, possibly because the virus was adsorbed to the leachate particulates. The masking effects associated with suspended solids in the leachate were overcome by adding a final 0.1 M sodium (tetra)ethylenediaminetetraacetate concentration to the leachate. A sodium (tetra)ethylenediaminetetraacetate-treated leachate could be clarified by filtration at pH 8.0 without a loss of virus. The clarified and sodium (tetra)ethylenediaminetetraacetate-treated leachate contained interfering materials of an anionic nature which prevented virus adsorption to epoxy-fiber glass filters. This interfering effect was overcome by treating the leachate with an anion-exchange resin. Viruses in the resin-treated leachate were concentrated by adjusting the leachate to pH 3.5, adding AlCl(3) to a final 0.005 M concentration, adsorbing the viruses to an epoxy-fiber glass virus adsorbent, and eluting the adsorbed viruses in a small volume. When this method was used to concentrate poliovirus 100-fold in a variety of leachates, the average virus recovery efficiency was 37%. With the methods described in this study, it should be possible to efficiently monitor solid waste disposal site leachates for enteric viruses.
已开发出用于检测和浓缩城市固体废弃物填埋场渗滤液中肠道病毒的方法。添加到渗滤液中的脊髓灰质炎病毒不易被检测到，可能是因为该病毒吸附在了渗滤液颗粒上。通过向渗滤液中添加终浓度为0.1M的（四）乙二胺四乙酸钠克服了与渗滤液中悬浮固体相关的掩盖效应。经（四）乙二胺四乙酸钠处理的渗滤液在pH 8.0时可通过过滤澄清，且病毒不会损失。澄清且经（四）乙二胺四乙酸钠处理的渗滤液含有阴离子性质的干扰物质，这些物质会阻止病毒吸附到环氧玻璃纤维滤器上。通过用阴离子交换树脂处理渗滤液克服了这种干扰效应。通过将树脂处理过的渗滤液的pH值调至3.5，添加AlCl₃使其终浓度达到0.005M，将病毒吸附到环氧玻璃纤维病毒吸附剂上，并在小体积中洗脱吸附的病毒，从而浓缩树脂处理过的渗滤液中的病毒。当使用该方法在多种渗滤液中将脊髓灰质炎病毒浓缩100倍时，病毒的平均回收效率为37%。采用本研究中所述的方法，应该能够有效地监测固体废弃物处置场渗滤液中的肠道病毒。
