小鼠白血病病毒诱导后独特DNA序列的存在。（RNA肿瘤病毒-核酸杂交-病毒DNA的插入）
The presence of unique DNA sequences after viral induction of leukemia in mice. (RNA tumor virus-nucleic acid hybridization-insertion of viral DNA).
作者信息
Sweet R W, Goodman N C, Cho J R, Ruprecht R M, Redfield R R, Spiegelman S
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1705-9. doi: 10.1073/pnas.71.5.1705.
From previous studies, lymphocyte DNA from human leukemias and DNA from involved tissues of patients with Hodgkin's disease or Burkitt's lymphoma contain sequences that are absent from their normal counterparts. These sequences are related to those found in particulate elements associated with these neoplasias and possessing biochemical properties characteristic of RNA tumor viruses. Similar observations have been made of unique sequences related to those of the feline virus RD-114 and found in spontaneous mastocytomas in cats. Here we extend these results to the classical murine model of virus-induced leukemias. Splenic DNA from BALB/c mice with leukemia induced by Rauscher leukemia virus (RLV) possess some RLV-related sequences that do not exist in normal BALB/c DNA. Furthermore, these leukemia-specific sequences were absent in all other mouse strains examined, including AKR, a strain with a high incidence of spontaneous leukemia. The DNA of all noninfected mouse strains possesses considerable homology with the RLV genome. Temperature denaturation studies indicate, however, that although the RLV-related sequences found in all normal mice are similar to each other, they are not exactly homologous with RLV sequences. We conclude that RLV-induced leukemia in BALB/c results in the insertion of RLV sequences into cellular DNA that itself possesses only partial homology with the RLV genome.
从先前的研究可知，人类白血病患者的淋巴细胞DNA以及霍奇金病或伯基特淋巴瘤患者受累组织的DNA中含有其正常对应物中不存在的序列。这些序列与在与这些肿瘤相关的颗粒成分中发现的序列有关，并且具有RNA肿瘤病毒的生化特性。在猫的自发性肥大细胞瘤中也发现了与猫病毒RD - 114相关的独特序列，并得到了类似的观察结果。在此，我们将这些结果扩展到病毒诱导白血病的经典小鼠模型。感染劳氏肉瘤病毒（RLV）诱导白血病的BALB/c小鼠的脾脏DNA含有一些正常BALB/c DNA中不存在的与RLV相关的序列。此外，在所检测的所有其他小鼠品系中，包括自发白血病发病率高的AKR品系，均不存在这些白血病特异性序列。所有未感染小鼠品系的DNA与RLV基因组具有相当的同源性。然而，温度变性研究表明，尽管在所有正常小鼠中发现的与RLV相关的序列彼此相似，但它们与RLV序列并非完全同源。我们得出结论，RLV诱导的BALB/c白血病导致RLV序列插入到细胞DNA中，而该细胞DNA本身与RLV基因组仅具有部分同源性。
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