禽成髓细胞瘤病毒RNA的双链化程度
Extent of double strandedness in avian myeloblastosis virus RNA.
作者信息
Cavalieri L F
出版信息
J Virol. 1974 Dec;14(6):1458-62. doi: 10.1128/JVI.14.6.1458-1462.1974.
Abstract
The extent of double strandedness of avian myeloblastosis virus 70S RNA has been determined from fluorescence measurements of the intercalation of ethidium bromide. We have shown that 50% of the nucleotides of 70S RNA in solution are in a stable helical configuration. This value does not include small helical regions that are too unstable to permit intercalation of the dye. The avian myeloblastosis virus RNA as it exists within the virion has the same degree of helicity as the free 70S RNA. Heating the free 70S RNA to 55 or 70 C, followed by cooling, does not measurably change the degree of helicity; the subunits therefore have as much helicity as the parent molecule.
摘要
通过对溴化乙锭嵌入的荧光测量，已确定禽成髓细胞瘤病毒70S RNA的双链程度。我们已经表明，溶液中70S RNA的50%核苷酸处于稳定的螺旋结构。该值不包括因过于不稳定而无法使染料嵌入的小螺旋区域。存在于病毒粒子内的禽成髓细胞瘤病毒RNA与游离的70S RNA具有相同程度的螺旋性。将游离的70S RNA加热到55或70℃，然后冷却，不会显著改变螺旋程度；因此，亚基与亲本分子具有同样多的螺旋性。
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