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通过阿的平荧光猝灭证明,功能性厌氧电子传递与大肠杆菌膜中硝酸盐和富马酸盐的还原有关。

Functional anaerobic electron transport linked to the reduction of nitrate and fumarate in membranes from Escherichia coli as demonstrated by quenching of atebrin fluorescence.

作者信息

Haddock B A, Kendall-Tobias M W

出版信息

Biochem J. 1975 Dec;152(3):655-9. doi: 10.1042/bj1520655.

Abstract

Measurements were made of energy-dependent quenching of atebrin fluorescence in membrane particles prepared from Escherichia coli grown anaerobically with glycerol as carbon source in the presence of either nitrate or fumarate. It is concluded that this technique can be used to study the functional organization of the anaerobic proton-translocating electron-transport chains that use nitrate or fumarate as terminal electron acceptor.

摘要

对在硝酸盐或富马酸盐存在的情况下,以甘油作为碳源厌氧培养的大肠杆菌制备的膜颗粒中疟涤平荧光的能量依赖性猝灭进行了测量。得出的结论是,该技术可用于研究以硝酸盐或富马酸盐作为末端电子受体的厌氧质子转运电子传递链的功能组织。

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