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轴丝在问号钩端螺旋体运动中的作用

Axial filament involvement in the motility of Leptospira interrogans.

作者信息

Bromley D B, Charon N W

出版信息

J Bacteriol. 1979 Mar;137(3):1406-12. doi: 10.1128/jb.137.3.1406-1412.1979.

DOI:10.1128/jb.137.3.1406-1412.1979
PMID:438121
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC218325/
Abstract

Motility mutants of Leptospira interrogans serovar illini were isolated and analyzed by dark-field and electron microscopy. Mutants were obtained by screening for small colonies after nitrosoguanidine treatment. One class of mutants did not have hook- or spiral-shaped ends. In addition, the axial filaments from these mutants were not coiled. An analysis of revertants of two of the mutants in this class indicated that the mutations were pleiotropic with respect to motility, hook- and spiral-shaped ends, and axial filament coiling. We conclude that the axial filaments and the hook- and spiral-shaped ends are involved in L. interrogans motility.

摘要

对问号钩端螺旋体血清型伊利尼的运动突变体进行了分离,并通过暗视野显微镜和电子显微镜进行分析。通过对亚硝基胍处理后的小菌落进行筛选获得突变体。一类突变体没有钩状或螺旋状末端。此外,这些突变体的轴丝没有盘绕。对该类中的两个突变体的回复体分析表明,这些突变在运动性、钩状和螺旋状末端以及轴丝盘绕方面是多效性的。我们得出结论,轴丝以及钩状和螺旋状末端参与了问号钩端螺旋体的运动。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/0be461827421/jbacter00286-0357-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/815a4a4fb500/jbacter00286-0354-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/bee778cf2293/jbacter00286-0355-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/64498a69520a/jbacter00286-0356-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/0be461827421/jbacter00286-0357-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/815a4a4fb500/jbacter00286-0354-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/bee778cf2293/jbacter00286-0355-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/64498a69520a/jbacter00286-0356-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ba9/218325/0be461827421/jbacter00286-0357-a.jpg

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本文引用的文献

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VIRULENCE-LINKED COLONIAL AND MORPHOLOGICAL VARIATION IN LEPTOSPIRA.钩端螺旋体中与毒力相关的菌落及形态变异
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A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete.一种新型鞭毛鞘蛋白FcpA决定了钩端螺旋体的细丝盘绕、平移运动和毒力。
Mol Microbiol. 2016 Aug;101(3):457-70. doi: 10.1111/mmi.13403. Epub 2016 May 24.
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Analysis of a Spontaneous Non-Motile and Avirulent Mutant Shows That FliM Is Required for Full Endoflagella Assembly in Leptospira interrogans.对一株自发的无运动性和无毒力突变体的分析表明,问号钩端螺旋体完整的内鞭毛组装需要FliM。
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Flagellar motility of the pathogenic spirochetes.致病性螺旋体的鞭毛运动
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