Durr I F, Habbal M Z
J Bacteriol. 1970 May;102(2):603-4. doi: 10.1128/jb.102.2.603-604.1970.
The supernatant fractions of lysates of Lactobacillus plantarum metabolize mevalonate into lipids. Adenosine triphosphate and uridine, as well as related compounds, and reduced nicotinamide adenine dinucleotide phosphate or reduced nicotinamide adenine dinucleotide stimulate this process. To obtain very active supernatant fractions, the method of lysis is modified to include polyamines during lysozyme treatment of cells and subsequent shocking with citrate buffer.
植物乳杆菌裂解物的上清液组分可将甲羟戊酸代谢为脂质。三磷酸腺苷、尿苷以及相关化合物，还有还原型烟酰胺腺嘌呤二核苷酸磷酸或还原型烟酰胺腺嘌呤二核苷酸可刺激这一过程。为获得活性极高的上清液组分，对裂解方法进行了改进，即在溶菌酶处理细胞期间加入多胺，并随后用柠檬酸盐缓冲液进行冲击处理。
