从大鼠肝脏苯丙氨酸转运核糖核酸中分离羟基-Y碱基。
Isolation of hydroxy-Y base from rat liver tRNAPhe.
作者信息
Kasai H, Yamaizumi Z, Kuchino Y, Nishimura S
出版信息
Nucleic Acids Res. 1979 Mar;6(3):993-9. doi: 10.1093/nar/6.3.993.
Abstract
A Y-base derivative was isolated from rat liver tRNAPhe and its structure was assigned to be alpha-(carboxyamino)-beta-hydroxy-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-a]purine-7-butyric acid dimethyl ester (hydroxy-Y), based on the results of mass spectrometry and chemical degradation. This modified base seems to be the major fluorescent component of rat liver tRNAPhe; the peroxy-Y base previously isolated from rat liver tRNAPhe and characterized by Nakanishi and his coworkers (1,2) was not present in our preparation.
摘要
从大鼠肝脏苯丙氨酸转运核糖核酸（tRNAPhe）中分离出一种Y碱基衍生物，基于质谱分析和化学降解结果，其结构被确定为α-（羧基氨基）-β-羟基-4,9-二氢-4,6-二甲基-9-氧代-1H-咪唑并[1,2-a]嘌呤-7-丁酸二甲酯（羟基-Y）。这种修饰碱基似乎是大鼠肝脏tRNAPhe的主要荧光成分；我们的制备物中不存在先前从大鼠肝脏tRNAPhe中分离并由中岸及其同事鉴定的过氧-Y碱基（1,2）。
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本文引用的文献
1
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J Am Chem Soc. 1968 Jul 17;90(15):4182-4. doi: 10.1021/ja01017a062.
2
Structure of the fluorescent Y base from yeast phenylalanine transfer ribonucleic acid.来自酵母苯丙氨酸转移核糖核酸的荧光Y碱基的结构。
J Am Chem Soc. 1970 Dec 30;92(26):7617-9. doi: 10.1021/ja00729a035.
3
Structure of the "peroxy-Y base" from liver tRNA Phe .来自肝脏苯丙氨酸转运核糖核酸的“过氧-Y碱基”结构
Nat New Biol. 1971 Nov 24;234(47):107-9. doi: 10.1038/newbio234107b0.
4
Chemical modification of the fluorescent base in phenylalanine transfer ribonucleic acid.苯丙氨酸转移核糖核酸中荧光碱基的化学修饰。
Biochemistry. 1971 Jul 20;10(15):2969-76. doi: 10.1021/bi00791a027.
5
Isolation and structure determination of the fluorescent base from bovine liver phenylalanine transfer ribonucleic acid.从牛肝苯丙氨酸转移核糖核酸中分离荧光碱基并确定其结构
Biochemistry. 1973 Jan 16;12(2):188-93. doi: 10.1021/bi00726a002.
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7
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