Kasai H, Goto M, Ikeda K, Zama M, Mizuno Y, Takemura S, Matsuura S, Sugimoto T, Goto T
Biochemistry. 1976 Feb 24;15(4):898-904. doi: 10.1021/bi00649a027.
A fluorescent base wye (Yt base) was isolated from Torulopsis utilis tRNAPhe. The structure was established as 4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazo[1,2-alpha]purine based on ultraviolet (uv), nuclear magnetic resonance (NMR), and mass spectra, and by direct comparison with synthetic material. The nucleoside, wyosine (Yt), was isolated from purified tRNAPhe by enzymatic degradations followed by column and thin-layer chromatographies. The structure of wyosine is proposed as 3-ribofuranosyl-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-alpha]purine (the ribosyl group is attached to the N-9 position of guanine nucleus) on the basis of comparison with model compounds.
从产朊假丝酵母苯丙氨酸tRNA中分离出一种荧光碱基怀（Yt碱基）。根据紫外（uv）、核磁共振（NMR）和质谱，并通过与合成材料直接比较，确定其结构为4,9-二氢-4,6-二甲基-9-氧代-1H-咪唑并[1,2-α]嘌呤。通过酶促降解，然后进行柱色谱和薄层层析，从纯化的苯丙氨酸tRNA中分离出核苷怀（Yt）。基于与模型化合物的比较，怀的结构被认为是3-呋喃核糖基-4,9-二氢-4,6-二甲基-9-氧代-1H-咪唑并[1,2-α]嘌呤（核糖基连接在鸟嘌呤核的N-9位）。
