利用31P核磁共振对肾脏中的代谢物和组织pH值进行无损测量。
Non-destructive measurement of metabolites and tissue pH in the kidney by 31P nuclear magnetic resonance.
作者信息
Sehr P A, Bore P J, Papatheofanis J, Radda G K
出版信息
Br J Exp Pathol. 1979 Dec;60(6):632-41.
Abstract
Phosphorus nuclear magnetic resonance (31P NMR) can be used as a non-destructive method for the simultaneous observation of the major phosphate-containing metabolites (ATP, ADP, nucleotide monophosphate, Pi, sugar phosphate) and intracellular pH in isolated rat kidney. The time course of changes in these metabolites and in cellular pH in the ischaemic kidney are examined at two temperatures and in the presence of different flushing media. ATP is rapidly depleted while the pH change is slower and shows biphasic behaviour. Pi production and total nucleotide (ATP and ADP) depletion also occur on the same time-scale as the tissue acidification. The relation of these observations to tissue viability is discussed and the possibility of extending the measurements to human organs is considered.
摘要
磷核磁共振（31P NMR）可作为一种非破坏性方法，用于同时观察分离的大鼠肾脏中主要含磷代谢物（三磷酸腺苷、二磷酸腺苷、核苷酸单磷酸、无机磷酸盐、磷酸糖）和细胞内pH值。在两种温度以及不同冲洗介质存在的情况下，研究了缺血肾脏中这些代谢物和细胞pH值的变化时间过程。三磷酸腺苷迅速消耗，而pH值变化较慢且呈双相行为。无机磷酸盐生成和总核苷酸（三磷酸腺苷和二磷酸腺苷）消耗也与组织酸化发生在同一时间尺度上。讨论了这些观察结果与组织活力的关系，并考虑了将测量扩展到人体器官的可能性。
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