Trudinger P A
J Bacteriol. 1970 Oct;104(1):158-70. doi: 10.1128/jb.104.1.158-170.1970.
The separation of an autoxidizable brown pigment, P582, from Desulfotomaculum nigrificans is described. It reacted with Na(2)S(2)O(4) and was characterized by absorption maxima in the oxidized state at 392, 582, and 700 nm. In the presence of Na(2)S(2)O(4), P582 formed complexes with CO and, under alkaline conditions, pyridine. There was no reaction with cyanide. The molecular weight of P582 was approximately 145,000, and the purest preparations contained Fe, Zn, and acid-labile sulfide but not Cu, Mo, or Mn. Preparations of P582 catalyzed the reduced methyl viologen (MVH)-linked reduction of sulfite, hydroxylamine, and nitrite but not of sulfate, thiosulfate, or nitrate. Reduced pyridine nucleotides did not substitute for MVH. A major product of the MVH-sulfite reaction was sulfide. CO partially inhibited the enzymatic activities. Sulfite, hydroxylamine, and nitrite and CO caused changes in the spectrum of Na(2)S(2)O(4)-reduced P582. Fe(2+)-chelating reagents reacted with part of the Fe of P582 and caused partial losses of labile sulfide and enzymatic activity. The spectral and CO-reacting properties of P582 were, however, unaffected by chelating agents. The reaction between P582 and chelating agents was stimulated by reducing agents.
本文描述了从黑化脱硫肠状菌中分离出一种可自动氧化的褐色色素P582的过程。它能与连二亚硫酸钠反应，其氧化态在392、582和700纳米处有吸收最大值。在连二亚硫酸钠存在的情况下，P582能与一氧化碳形成络合物，在碱性条件下还能与吡啶形成络合物。它与氰化物不发生反应。P582的分子量约为145,000，最纯的制剂含有铁、锌和酸不稳定硫化物，但不含铜、钼或锰。P582制剂能催化还原型甲基紫精（MVH）介导的亚硫酸盐、羟胺和亚硝酸盐的还原反应，但不能催化硫酸盐、硫代硫酸盐或硝酸盐的还原反应。还原型吡啶核苷酸不能替代MVH。MVH-亚硫酸盐反应的主要产物是硫化物。一氧化碳部分抑制酶活性。亚硫酸盐、羟胺、亚硝酸盐和一氧化碳会导致连二亚硫酸钠还原的P582光谱发生变化。铁（Ⅱ）螯合剂与P582的部分铁发生反应，导致不稳定硫化物和酶活性部分丧失。然而，P582的光谱和与一氧化碳反应的特性不受螯合剂影响。还原剂会促进P582与螯合剂之间的反应。
