Hecker D, Klug H, Tanneberger S, Rose H
Arch Geschwulstforsch. 1974;44(3):195-211.
Histochemical and morphological methods are reported to test the sensitivity of organ culture of human and animal tumors to cytostatics. Topochemical investigations of lactate-dehydrogenase and NADH-diaphorase in organ culture of UV-induced mice tumors have shown that there is a considerably decreased enzyme activity, as compared with the controls, after application of a clinical as well as a tenfold clinical dose, especially after treatment with trenimon and 5-fluorurazil. On the other hand a decreased activity of these enzymes was found in organ cultures of tumors induced by methylcholanthren, following application of vinblastin and 5-fluorurazil. Three out of five of the investigated mammary carcinomas exhibited a markedly decreased enzyme activity when tested with cytostatics. Furthermore, a significant decrease of the enzyme activity was observed in two ovarian carcinomas and in two malignant melanomas of three tumors examined in each case. Following the administration of cytostatics, electronmicroscopical studies showed marked changes in the mitochondria in mammary carcinomas as well as in ovarian carcinomas, whereas no alterations were found in melanomas. The findings are discussed in the light of individual chemotherapy of cancer.
据报道，组织化学和形态学方法可用于检测人和动物肿瘤器官培养物对细胞抑制剂的敏感性。对紫外线诱导的小鼠肿瘤器官培养物中乳酸脱氢酶和NADH-黄递酶的拓扑化学研究表明，与对照组相比，在应用临床剂量以及十倍临床剂量后，尤其是在用三胺硫磷和5-氟尿嘧啶治疗后，酶活性显著降低。另一方面，在用长春碱和5-氟尿嘧啶处理后，在甲基胆蒽诱导的肿瘤器官培养物中发现这些酶的活性降低。在所研究的五例乳腺癌中，有三例在用细胞抑制剂检测时显示酶活性明显降低。此外，在每例检查的三例肿瘤中的两例卵巢癌和两例恶性黑色素瘤中观察到酶活性显著降低。在用细胞抑制剂给药后，电子显微镜研究显示乳腺癌和卵巢癌中的线粒体有明显变化，而黑色素瘤中未发现改变。根据癌症的个体化化疗对这些发现进行了讨论。
