Hecker D, Deutschmann A
Acta Histochem. 1976;55(1):8-13.
Organ cultures of malignant tumours were histochemically and electronmicroscopically investigated. There was established that follows enzymes show a little activity in cultured tumour cells after 24 and 48 h: succinate dehydrogenase, alkaline phosphatase, adenosine triphosphatase, and nonspecific esterase, whereas NADH-diophorase, lactate dehydrogenase, and acid phosphatase show an essentially higher activity after termination of the cultivation. However, in comparison with the primare tissue, the activities of the last mentioned enzymes are clearly decreased in cultured tumour cells after termination of the cultivation. No changes of cell structures have electronmicroscopically been observed on these cultures of malignant tumours.
对恶性肿瘤的器官培养物进行了组织化学和电子显微镜研究。结果表明，琥珀酸脱氢酶、碱性磷酸酶、三磷酸腺苷酶和非特异性酯酶在培养24小时和48小时后的肿瘤细胞中活性较低，而烟酰胺腺嘌呤二核苷酸脱氢酶、乳酸脱氢酶和酸性磷酸酶在培养结束后活性明显较高。然而，与原发组织相比，培养结束后培养的肿瘤细胞中上述最后几种酶的活性明显降低。在这些恶性肿瘤培养物中，电子显微镜未观察到细胞结构的变化。
