Doiron D R, Profio E, Vincent R G, Dougherty T J
Chest. 1979 Jul;76(1):27-32. doi: 10.1378/chest.76.1.27.
A system using the fluorescence bronchoscope has been designed for localization of small, early bronchogenic carcinoma by the fluorescence of previously injected hematoporphyrin derivative. The system included a 200W mercury vapor lamp and primary filter, flexible fiberoptic bronchoscope with special violet-transmitting light conductor, secondary filter, and image intensifier tube. Tests indicated the system could detect a tumor only 100 micron thick at the expected concentration of hematoporphyrin derivative: 1 microgram/gm at 48 to 96 hours following intravenous injection at a dosage of 2.5 mg/kg. Examination of resected specimens (six of lung, one of esophagus) showed positive fluorescence in all cases, with fluorescence visible beyond the region visible under conventional white light examination. Fluorescence bronchoscopy has been performed on four patients thus far. Positive fluorescence was observed in all three cases where the tumor had been known to occur. Positive fluorescence was also observed in the patient with sputum positive for lung cancer, but negative x-ray film findings. However, additional examinations are required to demonstrate the smallest lesion that can be detected in vivo.
一种利用荧光支气管镜的系统已被设计出来，用于通过先前注射的血卟啉衍生物的荧光来定位小的早期支气管源性癌。该系统包括一盏200瓦的汞蒸气灯和初级滤光片、带有特殊透紫光光导的柔性纤维支气管镜、次级滤光片以及影像增强管。测试表明，在静脉注射剂量为2.5毫克/千克后48至96小时，在预期的血卟啉衍生物浓度为1微克/克时，该系统能够检测出仅100微米厚的肿瘤。对切除标本（六个肺部标本、一个食管标本）的检查显示，所有病例均有阳性荧光，且荧光可见范围超出了传统白光检查下可见的区域。到目前为止，已对四名患者进行了荧光支气管镜检查。在已知有肿瘤发生的所有三例病例中均观察到阳性荧光。在痰检肺癌阳性但X光片检查结果为阴性的患者中也观察到了阳性荧光。然而，需要进一步的检查来证明在体内能够检测到的最小病变。
