由I-EC亚区决定的两种同源但不相同的Ia分子的证据。
Evidence for two homologous, but nonidentical, Ia molecules determined by the I-EC subregion.
作者信息
Delovitch T L, Barber B H
出版信息
J Exp Med. 1979 Jul 1;150(1):100-7. doi: 10.1084/jem.150.1.100.
Abstract
Sequential immunoprecipitation, two-dimensional gel electrophoresis and peptide mapping analyses of B10A(3R), 35S-methionine-labeled, I-EC subregion products were performed. Evidence is presented here for the presence of two structurally homologous, but nonidentical, gene products of the I-EC subregion. These two Ia molecules are independently immunoprecipitable, identical in molecular size and charge, but differ by approximately equal to 20% in their peptides obtained by partial digestion with Staphylococcus aureus protease V8.
摘要
对用35S-甲硫氨酸标记的B10A(3R) I-EC亚区产物进行了顺序免疫沉淀、二维凝胶电泳和肽图谱分析。本文提供的证据表明，I-EC亚区存在两种结构同源但不相同的基因产物。这两种Ia分子可独立进行免疫沉淀，分子大小和电荷相同，但用金黄色葡萄球菌蛋白酶V8部分消化得到的肽段约有20%的差异。
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