Stites D P, Carr M C, Fudenberg H H
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1440-4. doi: 10.1073/pnas.69.6.1440.
The reactivity of cells in vitro was investigated with specimens from various lymphoid organs of seven human fetuses. Thymocytes responded to stimulation by phytohemagglutinin with significant increases in synthesis of DNA, but failed to produce destruction of xenogeneic target cells. In cells from bone marrow, precisely the converse pattern of reactivity to the mitogen was detected. Lymphocytes from spleen and peripheral blood demonstrated both phytohemagglutinin-dependent functions, while hepatic cells did not respond to phytohemagglutinin. Based on the striking dichotomy of phytohemagglutinin-dependent responses in fetal thymocytes and bone-marrow lymphoid cells, we conclude that phytohemagglutinin-dependent cytotoxicity and DNA synthesis are functions of different populations of lymphoid cells during human embryonic development.
利用来自7例人类胎儿不同淋巴器官的标本，对细胞在体外的反应性进行了研究。胸腺细胞对植物血凝素的刺激有反应，DNA合成显著增加，但未能破坏异种靶细胞。在来自骨髓的细胞中，检测到对促细胞分裂剂的反应模式恰恰相反。脾脏和外周血中的淋巴细胞表现出两种依赖植物血凝素的功能，而肝细胞对植物血凝素无反应。基于胎儿胸腺细胞和骨髓淋巴细胞中依赖植物血凝素反应的显著二分法，我们得出结论，在人类胚胎发育过程中，依赖植物血凝素的细胞毒性和DNA合成是不同淋巴细胞群体的功能。
