黄素1，N⁶-乙烯腺嘌呤二核苷酸：荧光的动态和静态猝灭
Flavin 1, N 6 -ethenoadenine dinucleotide: dynamic and static quenching of fluorescence.
作者信息
Barrio J R, Tolman G L, Leonard N J, Spencer R D, Weber G
出版信息
Proc Natl Acad Sci U S A. 1973 Mar;70(3):941-3. doi: 10.1073/pnas.70.3.941.
Abstract
Flavin 1,N(6)-ethenoadenine dinucleotide (epsilonFAD) was prepared by the action of chloroacetaldehyde on flavin adenine dinucleotide. This compound, which has two potential fluorophores, epsilon-adenine and isoalloxazine, shows extremely efficient energy transfer from the former to the latter. The fluorescences of both moieties are greatly diminished in the intact molecule. Determination of the fluorescence yields and lifetimes leads to the conclusion that at 20 degrees in neutral aqueous solution epsilonFAD exists mainly (90%) as an internally complexed or stacked form.
摘要
黄素1，N(6)-乙烯腺嘌呤二核苷酸（εFAD）是由氯乙醛作用于黄素腺嘌呤二核苷酸制备而成的。该化合物有两个潜在的荧光团，即ε-腺嘌呤和异咯嗪，显示出从前一个荧光团到后一个荧光团的极其高效的能量转移。在完整分子中，两个部分的荧光都大大减弱。对荧光产率和寿命的测定得出结论，在20摄氏度的中性水溶液中，εFAD主要（90%）以内部复合或堆积形式存在。
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