Weber G, Tanaka F, Okamoto B Y, Drickamer H G
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1264-6. doi: 10.1073/pnas.71.4.1264.
The effect of pressure to 10 kilobars on the fluorescence characteristics of flavin mononucleotide (FMN), flavin-adenine dinucleotide (FAD), and on complexes of FMN with adenylic acid (AMP), and with I(-) has been studied. The properties measured include peak location, fluorescence yield, and lifetime. From these results the equilibrium constant K and the rate constant k(+) (*) for complex formation were evaluated as a function of pressure. The pressure dependence of these coefficients shows that the volume of the system decreases upon complex formation and that there is an expansion upon formation of the activated complex (DeltaVdouble dagger is positive). The implications of these results for protein denaturation are mentioned.
研究了10千巴压力对黄素单核苷酸（FMN）、黄素腺嘌呤二核苷酸（FAD）以及FMN与腺苷酸（AMP）和I⁻形成的复合物荧光特性的影响。所测量的性质包括峰位置、荧光产率和寿命。根据这些结果，评估了作为压力函数的复合物形成的平衡常数K和速率常数k(+) (*)。这些系数对压力的依赖性表明，复合物形成时系统体积减小，而活化复合物形成时体积膨胀（ΔV双匕首为正）。文中提到了这些结果对蛋白质变性的影响。
