由克隆的质粒CAM-OCT基因介导的诱变增加：假单胞菌属物种扩大底物范围的潜力
Increased mutagenesis mediated by cloned plasmid CAM-OCT genes: potential for expanding substrate ranges of Pseudomonas spp.
作者信息
McBeth D L, Hauer B
机构信息
Department of Microbiology and Immunology, CUNY Medical School/Sophie Davis School of Biomedical Education, New York 10031, USA.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3538-40. doi: 10.1128/aem.62.9.3538-3540.1996.
Abstract
Twenty-five kilobases of Pseudomonas plasmid CAM-OCT DNA encoding a DNA repair gene(s) was cloned into the broad-host-range vector pVK100. The presence of the cloned genes increased the isolation frequency of Pseudomonas putida derivatives capable of using ethyl lactate or 3-methyl-3-buten-1-ol as their carbon source 15- and 8-fold, respectively, after UV irradiation. Ethyl lactate-utilizing strains expressed a novel intracellular hydrolase.
摘要
将编码一个或多个DNA修复基因的25千碱基假单胞菌质粒CAM - OCT DNA克隆到广宿主范围载体pVK100中。克隆基因的存在使恶臭假单胞菌衍生物在紫外线照射后能够分别以乳酸乙酯或3 - 甲基 - 3 - 丁烯 - 1 - 醇作为碳源的分离频率提高了15倍和8倍。利用乳酸乙酯的菌株表达了一种新型细胞内水解酶。
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Increased mutagenesis mediated by cloned plasmid CAM-OCT genes: potential for expanding substrate ranges of Pseudomonas spp.由克隆的质粒CAM-OCT基因介导的诱变增加：假单胞菌属物种扩大底物范围的潜力
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