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由克隆的质粒CAM-OCT基因介导的诱变增加：假单胞菌属物种扩大底物范围的潜力

Increased mutagenesis mediated by cloned plasmid CAM-OCT genes: potential for expanding substrate ranges of Pseudomonas spp.

作者信息

McBeth D L, Hauer B

机构信息

Department of Microbiology and Immunology, CUNY Medical School/Sophie Davis School of Biomedical Education, New York 10031, USA.

出版信息

Appl Environ Microbiol. 1996 Sep;62(9):3538-40. doi: 10.1128/aem.62.9.3538-3540.1996.

DOI:10.1128/aem.62.9.3538-3540.1996
PMID:8795249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC168155/
Abstract

Twenty-five kilobases of Pseudomonas plasmid CAM-OCT DNA encoding a DNA repair gene(s) was cloned into the broad-host-range vector pVK100. The presence of the cloned genes increased the isolation frequency of Pseudomonas putida derivatives capable of using ethyl lactate or 3-methyl-3-buten-1-ol as their carbon source 15- and 8-fold, respectively, after UV irradiation. Ethyl lactate-utilizing strains expressed a novel intracellular hydrolase.

摘要

将编码一个或多个DNA修复基因的25千碱基假单胞菌质粒CAM - OCT DNA克隆到广宿主范围载体pVK100中。克隆基因的存在使恶臭假单胞菌衍生物在紫外线照射后能够分别以乳酸乙酯或3 - 甲基 - 3 - 丁烯 - 1 - 醇作为碳源的分离频率提高了15倍和8倍。利用乳酸乙酯的菌株表达了一种新型细胞内水解酶。

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[Localization of camphor degradative plasmids on the chromosome of Pseudomonas putida strains PaW].[恶臭假单胞菌菌株PaW染色体上樟脑降解质粒的定位]
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本文引用的文献

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Synthetic and biological catalysts in chemical synthesis: how to assess practical utility.化学合成中的合成催化剂与生物催化剂：如何评估实际效用。
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Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.大肠杆菌中的诱变作用及对脱氧核糖核酸损伤的诱导反应
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